ZFIN ID: ZDB-FIG-111215-44
Li et al., 2011 - Abca12-mediated lipid transport and Snap29-dependent trafficking of lamellar granules are crucial for epidermal morphogenesis in a zebrafish model of ichthyosis. Disease models & mechanisms   4(6):777-85 Full text @ Dis. Model. Mech.
Anatomical Terms:
Stage Range: 1-cell to Days 7-13

Fig. 2 abca12 and snap29 gene expression in normal zebrafish. (A–C) Zebrafish embryos were collected at 0 and 6 hpf and 1–8 dpf, and total RNA was isolated and cDNA prepared. The abca12 (A) and snap29 (C) mRNA expression levels were measured by RT-PCR and standardized against the mRNA expression level of the β-actin gene. (B) Whole-mount in situ hybridization of embryos at different stages of early development for abca12 expression; gastrula period (left panel), 24 hpf (right panel).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
abca12 WT standard conditions 1-cell Not Detected whole organism RTPCR
50%-epiboly to 90%-epiboly EVL ISH
Shield to Days 7-13 whole organism RTPCR
Prim-5 mucus secreting cell ISH
Prim-5 olfactory placode ISH
snap29 WT standard conditions Prim-5 Not Detected whole organism RTPCR
Long-pec to Days 7-13 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.