ZFIN ID: ZDB-FIG-111128-30
Wang et al., 2011 - Prdm1a and miR-499 act sequentially to restrict Sox6 activity to the fast-twitch muscle lineage in the zebrafish embryo. Development (Cambridge, England)   138(20):4399-404 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 6

Fig. 3

Derepression of sox6 transcription but not translation in slow-twitch fibres. (A-C) Optical sagittal sections of posterior trunk region of Tg(5.7 kb sox6:EGFP)i253; Tg(9.7 kb smyhc1:lyn-tdTomato)i261 zebrafish larvae. At 2 dpf (A), EGFP expression is restricted exclusively to fast-twitch fibres (green) lying beneath the superficial slow-twitch fibres (red). At 4 dpf (B), EGFP expression is detectable in a few slow-twitch fibres (arrowheads). By 6 dpf (C), all slow-twitch fibres express EGFP. (D-G) Transverse cryostat sections of Tg(BACsmyhc1:GFP)i108 (D,F) or Tg(9.7 kb smyhc1:lyn-tdTomato)i261 (E,G) embryos/larvae hybridised with a probe for sox6 mRNA (red) or stained with anti-Sox6 antibody (green), respectively. Arrows indicate slow-twitch fibre nuclei. (H-L) Transverse cryostat sections of 6 dpf larvae carrying the 5.7 kb sox6:EGFP reporter constructs with differing 3′UTRs, as illustrated in L. (H) Expression of EGFP driven by the 5.7 kb reporter construct with the SV40 3′UTR in slow-twitch fibres, as identified by mABF59 staining (red), is repressed by replacement of the SV40 3′UTR with the endogenous sox6 3′UTR (I). Mutation (J) or deletion (K) of the five putative miR-499 target sites restores ectopic EGFP expression in slow-twitch fibres. Note the increased levels of EGFP expression caused by deletion of the miR-499 sites (K). Scale bars: 10 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP i108Tg standard conditions Prim-5 slow muscle cell IFL
Day 6 slow muscle cell IFL
i253Tg standard conditions Long-pec trunk musculature fast muscle cell IFL
Long-pec Not Detected trunk musculature slow muscle cell IFL
Day 4 trunk musculature fast muscle cell IFL
Day 4 trunk musculature slow muscle cell IFL
Day 6 trunk musculature fast muscle cell IFL
Day 6 trunk musculature slow muscle cell IFL
i258Tg standard conditions Day 6 fast muscle cell IFL
i259Tg standard conditions Day 6 fast muscle cell IFL
Day 6 slow muscle cell IFL
i260Tg standard conditions Day 6 fast muscle cell IFL
Day 6 slow muscle cell IFL
sox6 i108Tg standard conditions Prim-5 fast muscle cell ISH
Day 6 slow muscle cell ISH
Ab1-sox6 i261Tg standard conditions Long-pec Not Detected slow muscle cell IHC
Ab1-sox6 Day 6 Not Detected slow muscle cell IHC
Tomato i261Tg standard conditions Long-pec slow muscle cell IFL
Day 6 slow muscle cell IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC i253Tg standard conditions Day 6 slow muscle cell
IHC i258Tg standard conditions Day 6 slow muscle cell
IHC i259Tg standard conditions Day 6 slow muscle cell
IHC i260Tg standard conditions Day 6 slow muscle cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development