PUBLICATION

Prdm1a and miR-499 act sequentially to restrict Sox6 activity to the fast-twitch muscle lineage in the zebrafish embryo

Authors

Wang, X., Ono, Y., Tan, S.C., Chai, R.J., Parkin, C., and Ingham, P.W.

ID

ZDB-PUB-110907-10

Date

2011

Source

Development (Cambridge, England) 138(20): 4399-404 (Journal)

Registered Authors

Ingham, Philip, Ono, Yosuke, Parkin, Caroline, Tan, Swee Chuan, Wang, Xingang

Keywords

none

MeSH Terms

Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Animals
Zebrafish/embryology*
Zebrafish/genetics
Zebrafish/metabolism*
Muscle Fibers, Slow-Twitch/metabolism
Muscle Fibers, Fast-Twitch/metabolism*
Nuclear Proteins/genetics
Nuclear Proteins/metabolism*
Muscle Development/genetics
Muscle Development/physiology
Animals, Genetically Modified
Gene Expression Regulation, Developmental
Base Sequence
Protein Biosynthesis
DNA-Binding Proteins/genetics
DNA-Binding Proteins/metabolism*
Oligodeoxyribonucleotides, Antisense/genetics
MicroRNAs/genetics
MicroRNAs/metabolism*

(all 21)

PubMed

21880783 Full text @ Development

Abstract

Sox6 has been proposed to play a conserved role in vertebrate skeletal muscle fibre type specification. In zebrafish, sox6 transcription is repressed in slow-twitch progenitors by the Prdm1a transcription factor. Here we identify sox6 cis-regulatory sequences that drive fast-twitch-specific expression in a Prdm1a-dependent manner. We show that sox6 transcription subsequently becomes derepressed in slow-twitch fibres, whereas Sox6 protein remains restricted to fast-twitch fibres. We find that translational repression of sox6 is mediated by miR-499, the slow-twitch-specific expression of which is in turn controlled by Prdm1a, forming a regulatory loop that initiates and maintains the slow-twitch muscle lineage.

Genes / Markers

Figures