ZFIN ID: ZDB-PUB-110907-10
Prdm1a and miR-499 act sequentially to restrict Sox6 activity to the fast-twitch muscle lineage in the zebrafish embryo
Wang, X., Ono, Y., Tan, S.C., Chai, R.J., Parkin, C., and Ingham, P.W.
Date: 2011
Source: Development (Cambridge, England)   138(20): 4399-404 (Journal)
Registered Authors: Ingham, Philip, Ono, Yosuke, Parkin, Caroline, Tan, Swee Chuan, Wang, Xingang
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism*
  • Gene Expression Regulation, Developmental
  • MicroRNAs/genetics
  • MicroRNAs/metabolism*
  • Muscle Development/genetics
  • Muscle Development/physiology
  • Muscle Fibers, Fast-Twitch/metabolism*
  • Muscle Fibers, Slow-Twitch/metabolism
  • Nuclear Proteins/genetics
  • Nuclear Proteins/metabolism*
  • Oligodeoxyribonucleotides, Antisense/genetics
  • Protein Biosynthesis
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 21880783 Full text @ Development
Sox6 has been proposed to play a conserved role in vertebrate skeletal muscle fibre type specification. In zebrafish, sox6 transcription is repressed in slow-twitch progenitors by the Prdm1a transcription factor. Here we identify sox6 cis-regulatory sequences that drive fast-twitch-specific expression in a Prdm1a-dependent manner. We show that sox6 transcription subsequently becomes derepressed in slow-twitch fibres, whereas Sox6 protein remains restricted to fast-twitch fibres. We find that translational repression of sox6 is mediated by miR-499, the slow-twitch-specific expression of which is in turn controlled by Prdm1a, forming a regulatory loop that initiates and maintains the slow-twitch muscle lineage.