FIGURE

Fig. 2

ID

ZDB-FIG-111028-5

Publication

Hale et al., 2011 - Netrin Signaling Breaks the Equivalence between Two Identified Zebrafish Motoneurons Revealing a New Role of Intermediate Targets

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Fig. 2

Ntn1a is necessary to prevent a second CaP/VaP axon from extending beyond the muscle pioneers.

Projected confocal stacks of living dye-labeled motoneurons in one spinal hemisegment. A. VaP axon stalled at muscle pioneers (dashed line) at 36 hpf in standard control-MO injected embryo. B. nt1a SBMO injected embryos had two CaP/VaP axons that extended beyond the muscle pioneers. One CaP/VaP cell also extended an ectopic branch (arrow) along the horizontal myoseptum. B′-B′′. The same cells as shown in B. At 25 hpf (B′) the red cell was a CaP, but by 31 hpf (B′′) the red cell had retracted its axon to the muscle pioneers intermediate target and the green cell had become CaP. Scale bars = 9 μm in A, B and 20 μm in B′, B′′.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-ntn1a
Knockdown Reagent:	MO2-ntn1a
Observed In:	muscle pioneer VaP motor neuron axon
Stage Range:	Prim-5 to Prim-25

Phenotype Detail

Acknowledgments

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