FIGURE

Fig. 1

ID

ZDB-FIG-111028-4

Publication

Hale et al., 2011 - Netrin Signaling Breaks the Equivalence between Two Identified Zebrafish Motoneurons Revealing a New Role of Intermediate Targets

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Ntn1a expressed in the muscle pioneers is a candidate signal for stopping VaP axon outgrowth.

A. Schematized view of one spinal hemisegment including spinal cord (sc) and overlying muscle. CaP (red) and VaP (green) axons contact muscle pioneers (blue) at the first intermediate target. The VaP axon stalls at the muscle pioneers while the CaP axon continues into ventral myotome (vm). ntn1a mRNA (yellow) is expressed in muscle pioneers [16]. This image and following images are oriented laterally with rostral to the left. B–C. Projected confocal stacks of (B) uninjected and (C) ntn1a SBMO-injected embryos labeled with F59, a slow muscle fiber marker [70]. At 24 hpf, muscle pioneers (green fibers in the middle of the images) are present in both control and MO-injected embryos. D. RT-PCR results confirming uninjected embryos have wildtype ntn1a (450 bp band) that is absent from embryos injected with 5.6 ng of ntn1a SBMO (star). Multiple samples were run in this gel. Lanes containing samples unrelated to ntn1a were cropped from the figure. Scale bar = 20 μm.

Expression Data

Antibody:	Ab-F59
Fish:	WT WT + MO2-ntn1a
Knockdown Reagent:	MO2-ntn1a
Anatomical Term:	muscle pioneer contractile muscle fiber
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One