FIGURE

Fig. S2

ID

ZDB-FIG-111014-20

Publication

Hammond et al., 2011 - Fgf and Hh signalling act on a symmetrical pre-pattern to specify anterior and posterior identity in the zebrafish otic placode and vesicle

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Fig. S2

Hair cell polarities in the entire medial macula of SU5402-treated embryos resemble those in the posterior macula of wild-type embryos. (A-L) Maculae of embryos treated with 10 μM SU5402 for either 3 or 5 hours from the 10S stage, stained at 86 hpf with anti-acetylated tubulin antibody to mark the kinocilia (red) and FITC-phalloidin to mark the actin-rich stereocilia (green) of each hair cell. (A,D,G,J) Confocal z-stacks showing overlaid tubulin and phalloidin stains. (B,E,H,K) Single channel z-stacks showing phalloidin stain only. (C,F,I,L) Polarity maps obtained from the phallodin/tubulin-stained specimens. Orientations are shown by arrows; a, anterior; v, ventral.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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