ZFIN ID: ZDB-FIG-110929-24
Greenhill et al., 2011 - An iterative genetic and dynamical modelling approach identifies novel features of the gene regulatory network underlying melanocyte development. PLoS Genetics   7(9):e1002265 Full text @ PLoS Genet.
Anatomical Terms:
Stage: Protruding-mouth

Fig. 7

Mitfa-dependent repression of sox10 expression in neural crest.

Whole-mount in situ hybridisation shows prominent expression of sox10 in peripheral glia and ear in mutants (D) and WT siblings (A); expression in WT melanocytes is undetectable (B), but mitfa mutants show prominent expression in many cells in the position of the dorsal stripe (E). (C, F) At this same stage expression of mitfa in WT siblings is undetectable under conditions used in this experiment (C), but can be shown by enhancing sensitivity by increasing PTU inhibition of melanisation and extending the signal development time (C, inset). Mitfa expression is clearly enhanced in mitfa mutants (F). Note that WTs have been treated with 0.00075% PTU to limit melanisation. B,C,E,F) dorsal views of posterior trunk, focused just above spinal cord. Scale bars, 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
sox10 WT chemical treatment: N-phenylthiourea Protruding-mouth glial cell ISH
Protruding-mouth inner ear ISH
mitfaw2/w2 standard conditions Protruding-mouth glial cell ISH
Protruding-mouth inner ear ISH
Protruding-mouth trunk cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
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