FIGURE

Fig. S5

ID

ZDB-FIG-110920-63

Publication

Bohnsack et al., 2011 - Phenothiourea Sensitizes Zebrafish Cranial Neural Crest and Extraocular Muscle Development to Changes in Retinoic Acid and IGF Signaling

Other Figures

All Figure Page

Back to All Figure Page

Fig. S5

T3 and T4 partially restore T4 expression in PTU-treated embryos. Wholemount immunostaining for T4 in 96 hpf embryos (ventral view) demonstrated that in the absence of PTU exogenous treatment with 100 nM T3 and 100 nM T4 between 6 and 96 hpf mildly decreased T4 expression in thyroid follicles (C) compared to embryos treated with T3 and T4 from 6 to 22 hpf (B) and untreated controls (A). Treatment with 0.003% (D) and 0.03% PTU (G) progressively decreased T4 expression compared to embryos raised in the absence of PTU (A). Treatment with exogenous T3 and T4 from 6 to 22 hpf (E) or from 6 to 96 hpf (F) did not improve the effect of 0.003% PTU on T4 expression (D) while exogenous T3 and T4 from 6 to 96 hpf (I), but not from 6 to 22 hpf (H) slightly improved T4 expression in embryos treated with 0.03% PTU (G).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One