FIGURE

Fig. 6

ID

ZDB-FIG-110920-57

Publication

Bohnsack et al., 2011 - Phenothiourea Sensitizes Zebrafish Cranial Neural Crest and Extraocular Muscle Development to Changes in Retinoic Acid and IGF Signaling

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Fig. 6

0.003% PTU Inhibits T4 in thyroid follicles.

Wholemount immunostaining for T4 in 96 hpf embryos (ventral view) raised in control media (A, B) or in media containing 0.003% PTU. PTU was added at 4, 8, 12, 16, 20, or 24 hpf and carried through until the embryos were harvested at 96 hpf. Untreated 96 hpf embryos showed numerous thyroid follicles that contained T4 (A, B). Addition of 0.003% PTU between 4 and 12 hpf suppressed T4 in follicles at 96 hpf (C, E). Addition of 0.003% PTU between 16 and 20 hpf progressively increases T4 staining and treatment at 24 hpf does not affect T4.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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