FIGURE

Fig. 5

ID

ZDB-FIG-110920-56

Publication

Bohnsack et al., 2011 - Phenothiourea Sensitizes Zebrafish Cranial Neural Crest and Extraocular Muscle Development to Changes in Retinoic Acid and IGF Signaling

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Fig. 5

PTU at high concentrations inhibits neural crest development.

96 hpf roy Tg(fli1::EGFP) and roy Tg(sox10::EGFP) embryos were treated with 0.003% (A?H) or 0.03% (I?P) PTU at 6 hpf or 22 hpf. Treatment with 0.003% PTU at 6 (A?D) or 22 (E?H) hpf inhibited pigmentation (A, E) but did not affect development of the retinal-hyaloid vasculature (B, F), pharyngeal arches (PA; C, G), and jaw cartilage (D, H) as compared to untreated embryos (Q?T). At a concentration of 0.03%, PTU at 6 hpf inhibited neural crest development (I?L) including pharyngeal arch development (K), jaw formation (L), and the remodeling of the hyaloid vessel into the hyaloids retinal plexus (J). Treatment with 0.03% PTU at 22 hpf caused pharyngeal arch (O) and jaw malformation (M, P), but did not affect the hyaloid-retinal vasculature (N).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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