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ZFIN ID: ZDB-FIG-110909-31
Johnson et al., 2011 - Vgll2a is required for neural crest cell survival during zebrafish craniofacial development. Developmental Biology   357(1):269-81 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-25
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-25

Fig. 3

Endodermal pouch morphogenesis is affected in vgll2a morphants. Single confocal, Z-plane images of an uninjected (A) and vgll2a morphant embryo (B), stained with the zn-8 antibody, marking the endodermal pouches in lateral views. Uninjected embryos (A) exhibit elongated endodermal pouches, numbered 1–5, formed by tight folds of epithelium at 38 hpf. In contrast, the endodermal pouches of embryos injected with 5 ng vgll2a ATG MO (B) are shorter and wider, and the epithelial cells appear less organized relative to uninjected controls. Anterior is to the left in both panels.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-8 IHC WT standard conditions Prim-25 pharyngeal pouch
IHC WT + MO2-vgll2a standard conditions Prim-25 pharyngeal pouch
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-vgll2a standard conditions Prim-25 pharyngeal epithelium disorganized, abnormal
Prim-25 pharyngeal pouch decreased length, abnormal
Prim-25 pharyngeal pouch increased width, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 357(1), Johnson, C.W., Hernandez-Lagunas, L., Feng, W., Melvin, V.S., Williams, T., and Artinger, K.B., Vgll2a is required for neural crest cell survival during zebrafish craniofacial development, 269-81, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.