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Fig. S2

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ZDB-FIG-110909-37

Publication

Johnson et al., 2011 - Vgll2a is required for neural crest cell survival during zebrafish craniofacial development

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Fig. S2

The pharyngeal cartilage phenotype of vgll2a morphants can be partially rescued by mRNA encoding vgll2a. Examples of 5 dpf larvae stained with alcian blue in ventral views (A–C) and quantification of the number of pharyngeal cartilage elements visible for each condition examined (D). In wildtype (A) the total number of pharyngeal cartilage elements at 5 dpf is normally 18 (n = 9). Embryos injected at the 1 cell stage with 100 pg vgll2aΔMO mRNA in addition to 6 ng vgll2a ATG MO injected at the 2 to 4 cell stage (n = 9) averaged 4.7 alcian-positive pharyngeal cartilage elements, significantly more (Student′s T test, *: p < 0.05) than those injected withvgll2a ATG MO alone (n = 8), which averaged 1.3. Error bars represent standard error of the mean. Anterior is to the left in A–C.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Developmental Biology, 357(1), Johnson, C.W., Hernandez-Lagunas, L., Feng, W., Melvin, V.S., Williams, T., and Artinger, K.B., Vgll2a is required for neural crest cell survival during zebrafish craniofacial development, 269-81, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.