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Fig. 3
Endodermal pouch morphogenesis is affected in vgll2a morphants. Single confocal, Z-plane images of an uninjected (A) and vgll2a morphant embryo (B), stained with the zn-8 antibody, marking the endodermal pouches in lateral views. Uninjected embryos (A) exhibit elongated endodermal pouches, numbered 1–5, formed by tight folds of epithelium at 38 hpf. In contrast, the endodermal pouches of embryos injected with 5 ng vgll2a ATG MO (B) are shorter and wider, and the epithelial cells appear less organized relative to uninjected controls. Anterior is to the left in both panels.
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Reprinted from Developmental Biology, 357(1), Johnson, C.W., Hernandez-Lagunas, L., Feng, W., Melvin, V.S., Williams, T., and Artinger, K.B., Vgll2a is required for neural crest cell survival during zebrafish craniofacial development, 269-81, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.