FIGURE

Fig. 2

ID

ZDB-FIG-110817-12

Publication

Neumann et al., 2011 - Mutation in the type IB bone morphogenetic protein receptor alk6b impairs germ-cell differentiation and causes germ-cell tumors in zebrafish

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Fig. 2

The tgct mutants exhibit impaired differentiation and reduced meiosis in vitro and in vivo. In wild-type testis (A), phosphohistone H2Ax marks clusters of primary spermatocytes undergoing meiosis (arrows). Spermatogonia (arrowheads) are phosphohistone H2AX-negative. The tgct mutant testis (B) exhibits severely reduced clusters of meiotic spermatocytes. The tumor cells are phosphohistone H2AX-negative. (C and D) In situ hybridization for the germ-cell–specific gene ziwi. In wild-type testis (C), ziwi expression is strong in clusters of spermatogonia (arrows) and rapidly declines as cells enter meiosis. The tgct testis tumors (D) show uniformly high expression of ziwi. (E) FACS analysis for DNA content of cultured wild-type and tgct testis on day 1. (F) FACS analysis on day 8 after culturing. Wild-type spermatogonia have completed meiosis to become haploid spermatocytes and spermatozoa (1N), but tgct tumor cells remain diploid (2N) and do not undergo meiosis. [Scale bars: 100 μm (A and B); 200 μm (C and D).]

Expression Data

Gene:	piwil1
Fish:	WT bmpr1bb^sw40/sw40
Anatomical Term:	spermatogonium
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	bmpr1bb^sw40/sw40
Observed In:	germ cell development male meiotic nuclear division sperm spermatocyte
Stage:	Adult

Phenotype Detail

Acknowledgments

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