FIGURE

Fig. 3

ID

ZDB-FIG-110817-13

Publication

Neumann et al., 2011 - Mutation in the type IB bone morphogenetic protein receptor alk6b impairs germ-cell differentiation and causes germ-cell tumors in zebrafish

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Fig. 3

Positional cloning of the tgct mutant locus. (A) Position of the tgct mutation on chromosome 10 between novel SSLP markers zT3 and zT5, located on Zv6 Ensembl scaffolds 1452 and 1450, respectively. (B) Chromatogram showing the G to A transition in alk6b^W256X mutants. (C) Schematic of Alk6b protein illustrating the Activin-like receptor (AcvR) ligand binding (LB), transmembrane (TM), GS box (GS), and serine/threonine kinase domains. The tgct mutation is located in the kinase domain at amino acid 256. (D) A β-actin-alk6b^wt transgene injected alk6b^W256X female showing no rescue of oocyte maturation and no transgene incorporation (E, Inset) into genomic DNA. (E) A β-actin-alk6b^wt transgene injected alk6b^W256^X female with restored oocyte maturation (*) and incorporation of the transgene into genomic DNA (Inset). (D and E: magnification 50×.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA