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ZFIN ID: ZDB-FIG-110804-80
Matsui et al., 2011 - Canopy1, a positive feedback regulator of FGF signaling, controls progenitor cell clustering during Kupffer's vesicle organogenesis. Proceedings of the National Academy of Sciences of the United States of America   108(24):9881-6 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Shield
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Shield

Fig. 3

FGF signaling plays crucial roles in DFC clustering and KV ciliogenesis. (A and B) sox32 (A) or no tail (B) expression in fgf8-MO–injected embryos. Dorsal view, anterior to the top. (Scale bar: 200 μm.) (A2 and B2) Higher-magnification images highlight DFCs. (B2, D, and E) The white dotted lines mark the boundary between DFCs and the blastoderm margin. (C) Percentages of normal or broken-up DFCs were scored by using the sox32 or no tail expression patterns in uninjected (n = 68 or 89) or fgf8-MO (n = 61 or 69) embryos. Statistically significant (P < 0.05) differences could be seen in uninjected versus fgf8-MO embryos. (DK) Transient activation of FGF signaling restored the broken-up DFC phenotype (D–F), ciliogenesis (G–J), and cardiac laterality (K) in DFCcnpy1-MO embryos. (D and E) Expression of no tail in DFCcnpy1-MO+iFGFR1 embryos treated with ethanol (D) or AP20187 (E). (F) Percentages of broken-up DFC phenotype in ethanol-treated (n = 84) or AP20187-treated (n = 93) DFCcnpy1-MO+iFGFR1 embryos. The conditional activation of Fgfr1 after treatment with AP20187 significantly decreased the broken-up DFC phenotype (67%; P < 0.05) (G–J) A-tubulin (green) staining in ethanol-treated (G) or AP20187-treated (H) DFCcnpy1-MO+iFGFR1 embryos at the six-somite stage. (Scale bar: 20 µm.) (I and J) Number (I) or length (J) of KV primary cilia in ethanol-treated DFCcnpy1-MO+iFGFR1 (n = 9 or 36) or AP20187-treated DFCcnpy1-MO+iFGFR1 (n = 8 or 34) embryos at the six-somite stage. (Error bars show SEM.) Statistically significant (P < 0.05) differences could be seen in ethanol-treated versus AP20187-treated DFCcnpy1-MO+iFGFR1 embryos. (K) Percentages of cardiac laterality defect in ethanol-treated (n = 89) or AP20187-treated (n = 102) DFCcnpy1-MO+iFGFR1 embryos. The conditional activation of Fgfr1 after treatment with AP20187 alleviated the cardiac laterality defect (48%; P < 0.05).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
sox32 WT + MO3-fgf8a standard conditions Shield forerunner cell group ISH
Shield presumptive endoderm ISH
tbxta WT + MO3-fgf8a standard conditions Shield forerunner cell group ISH
Shield margin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-fgf8a standard conditions Shield forerunner cell group distributed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA