FIGURE

Fig. 2

ID
ZDB-FIG-110804-60
Publication
Saito et al., 2011 - Isolation and cytogenetic characterization of zebrafish meiotic prophase I mutants
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Characterization of premeiotic germ cells in the its, isa, and imo zebrafish mutants. A–D: Immunohistochemical analysis of Plzf. Fresh-frozen sections of wild-type (A), its (B), isa (C), and imo (D) testes were stained with anti-Plzf antibodies (green). The nuclei stained with TOPRO-3 are visible in a magenta pseudo-color. Plzf-expressing male zebrafish germ cell populations, which contain type A spermatogonia and early type B spermatogonia, were normal in terms of cell number and distribution patterns in each mutant. E–H: Analysis of cell proliferation by BrdU (5-bromo-22-deoxy-uridine) incorporation. Zebrafish were labeled with BrdU for 2 hr and testicular paraffin sections were subsequently stained with anti-BrdU antibodies and counterstained with periodic acid–Schiff. In the wild-type zebrafish testis, cysts of BrdU-positive type B spermatogonia, which are premeiotic spermatogenic cells, were observed. In the testis of the its (F), isa (G), and imo (H) mutants, the incorporation of BrdU in type B spermatogonia were also observed. Scale bars = 100 μm.

Expression Data
Gene:
Fish:
Anatomical Term:
Stage: Adult

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Adult

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.