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ZIRC
ZFIN ID: ZDB-FIG-110726-2
Anastasaki et al., 2011 - Dhx34 and Nbas function in the NMD pathway and are required for embryonic development in zebrafish. Nucleic acids research   39(9):3686-3694 Full text @ Nucleic Acids Res.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5

Fig. 5

Both Dhx34 and Nbas are required for the in vivo degradation of the nonsense slc25a5 transcript in zebrafish. Expression of slc25a5 mRNA was measured in wild-type (A) and goldenb1 mutant (B) uninjected embryos, or embryos injected with indicated MOs, by real-time RT-PCR. The level of slc25a5 transcript was normalized to dct mRNA. The normalized values were then divided by values for uninjected samples (no MO) and expressed as relative mRNA levels.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
slc24a5 WT control Prim-5 whole organism RTPCR
WT + MO1-upf1 standard conditions Prim-5 whole organism RTPCR
WT + MO2-dhx34 standard conditions Prim-5 whole organism RTPCR
WT + MO2-nbas standard conditions Prim-5 whole organism RTPCR
slc24a5b1/b1 control Prim-5 whole organism RTPCR
slc24a5b1/b1 + MO1-upf1 standard conditions Prim-5 whole organism RTPCR
slc24a5b1/b1 + MO2-dhx34 standard conditions Prim-5 whole organism RTPCR
slc24a5b1/b1 + MO2-nbas standard conditions Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Nucleic acids research for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nucleic Acids Res.