FIGURE

Fig. 8

ID

ZDB-FIG-110720-29

Publication

Follo et al., 2011 - Knock-down of cathepsin D affects the retinal pigment epithelium, impairs swim-bladder ontogenesis and causes premature death in zebrafish

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Fig. 8

Immunofluorescence staining of microvilli in the eye of zebrafish following cathepsin D knock-down and rescue.

Immunofluorescence staining of α-actin (red) in eye sections derived from 4 dpf micro-injected larvae (CTRL = control injections; S-MPO = splicing morpholino; T-MPO = translation morpholino; RESCUED = translation morpholino plus 200 pg/egg of mutant CD mRNA). Nuclei are stained with DAPI (blue). The arrow points to microvilli of RPE cells. Note the absence of this structure in T-MPO zenbrafish. The photoreceptor cell (PRC) layer is indicated by curly brackets. Scale bar is 10 μm. Images representative of five (three for Rescued) independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ctsd WT + MO2-ctsd
Knockdown Reagents:	MO1-ctsd MO2-ctsd
Observed In:	retina retinal pigmented epithelium microvillus
Stage:	Day 4

Phenotype Detail

Acknowledgments

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