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ZFIN ID: ZDB-FIG-110622-22
Cederlund et al., 2011 - mab21l2 transgenics reveal novel expression patterns of mab21l1 and mab21l2, and conserved promoter regulation without sequence conservation. Developmental dynamics : an official publication of the American Association of Anatomists   240(4):745-754 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Adult

Fig. 4

Comparison of the ocular expression of mab21l1, mab21l2, and Tg(7.2mab2ll2:EGFP)ucd2. In <24 hpf eye sections, Tg(7.2mab2ll2:EGFP)ucd2 expresses EGFP in the lens (B), partly recapitulating mab21l2 (A), but not mab21l1, which is widely expressed in the retina (C). At 3 dpf, mab21l2 is weakly expressed in the peripheral INL, dorsal ciliary marginal zone, and ventral iridocorneal canal (D), whereas mab21l1 is upregulated in amacrine and ganglion cell layers (F). From 3 dpf to adult, Tg(7.2mab2ll2:EGFP) is expressed in a subpopulation of amacrine cells in the INL (E,H,K) and thereby partly recapitulates a prominent expression domain of mab21l1 (I,L). In contrast, mab21l2 is only expressed in the ventral iridocorneal canal at <5 dpf (G) and is undetectable in adult sections (J). mab21l1 and mab21l2 expression levels assayed by RT-PCR of 5-dpf eyes. At 5 dpf, mab21l1 is expressed at higher levels than mab21l2. Duplicate RNA samples were isolated at 9 am and 3 pm to confirm that transcription levels did not change during the day. Actin was used as a control (M). mab21l1 and mab21l2 expression levels assayed by microarray analysis of 3–5-dpf eyes This confirmed higher expression levels in the eye of mab21l1 in relation to mab21l2at 3–5 dpf, with decreasing mab21l2 transcript levels after 3 dpf (N). Arrow (A,C), lens; arrowhead (A,C), mab21l1/mab21l2 expression domains; Arrows (D), mab21l2-expressing cells; a, amacrine cell layer; g, ganglion cell layer; cmz, ciliary marginal zone; vic, iridocorneal canal; opl, outer plexiform layer; onl, outer nuclear layer; inl, inner nuclear layer; ipl, inner plexiform layer; gcl, ganglion cell layer.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP ucd2Tg control Prim-5 solid lens vesicle IFL
Protruding-mouth amacrine cell IFL
Protruding-mouth Not Detected lens IFL
Day 5 amacrine cell IFL
Adult amacrine cell IFL
mab21l1 WT control Prim-5 optic cup ISH
Protruding-mouth amacrine cell ISH
Protruding-mouth retinal ganglion cell ISH
Day 5 amacrine cell ISH
Day 5 ciliary marginal zone ISH
Day 5 eye RTPCR
Day 5 retinal ganglion cell ISH
Adult amacrine cell ISH
mab21l2 WT control Prim-5 optic cup ISH
Prim-5 solid lens vesicle ISH
Protruding-mouth ciliary marginal zone dorsal region ISH
Protruding-mouth Not Detected lens ISH
Protruding-mouth retinal inner nuclear layer ISH
Day 5 eye RTPCR
Day 5 eye ISH
Adult Not Detected whole organism ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.