PUBLICATION
mab21l2 transgenics reveal novel expression patterns of mab21l1 and mab21l2, and conserved promoter regulation without sequence conservation
- Authors
- Cederlund, M.L., Vendrell, V., Morrissey, M.E., Yin, J., Gaora, P.Ó., Smyth, V.A., Higgins, D.G., and Kennedy, B.N.
- ID
- ZDB-PUB-110317-10
- Date
- 2011
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 240(4): 745-754 (Journal)
- Registered Authors
- Cederlund, Maria, Kennedy, Breandan N., Yin, Jun
- Keywords
- eye, retina, promoter, zebrafish, amacrine, mab21l1, mab21l2
- MeSH Terms
-
- Amacrine Cells/metabolism
- Amacrine Cells/physiology
- Animals
- Animals, Genetically Modified
- Base Sequence
- Conserved Sequence*
- Embryo, Nonmammalian
- Eye/embryology
- Eye/metabolism
- Gene Expression Regulation, Developmental
- Homeodomain Proteins/genetics*
- Homeodomain Proteins/metabolism
- Homeodomain Proteins/physiology
- Molecular Sequence Data
- Promoter Regions, Genetic*
- Sequence Homology, Nucleic Acid
- Tissue Distribution
- Transgenes
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology
- PubMed
- 21360786 Full text @ Dev. Dyn.
Citation
Cederlund, M.L., Vendrell, V., Morrissey, M.E., Yin, J., Gaora, P.Ó., Smyth, V.A., Higgins, D.G., and Kennedy, B.N. (2011) mab21l2 transgenics reveal novel expression patterns of mab21l1 and mab21l2, and conserved promoter regulation without sequence conservation. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(4):745-754.
Abstract
mab21l1 and mab21l2 paralogs have widespread and dynamic expression patterns during vertebrate development. Both genes are expressed in the developing eye, midbrain, neural tube, and branchial arches. Our goal was to identify promoter regions with activity in mab21l2 expression domains. Assays of mab21l2 promoter-EGFP constructs in zebrafish embryos confirm that constructs containing 7.2 or 4.9 kb of mab21l2 promoter region are sufficient to drive expression in known (e.g., tectum, branchial arches) and unexpected domains (e.g., lens and retinal amacrine cells). A comparative analysis identifies complementary and novel expression domains of endogenous mab21l2 (e.g., lens and ventral iridocorneal canal) and mab21l1 (e.g., retinal amacrine and ganglion cells). Interestingly, therefore, despite the absence of conserved non-coding elements, a 4.9-kb mab21l2 promoter is sufficient to recapitulate expression in tissues unique to mab21l1 or mab21l2.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping