ZFIN ID: ZDB-FIG-110608-15
Quillien et al., 2011 - BMP signaling orchestrates photoreceptor specification in the zebrafish pineal gland in collaboration with Notch. Development (Cambridge, England)   138(11):2293-2302 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Term:
Stage: Long-pec

Fig. 3 BMP activity is sufficient to promote the PhR fate. (A-C) Confocal sections of control, Tg(hsp70:bmp2b) transgenic and Tg(hs:Gal4);Tg(UAS:caBmpr1a) double transgenic embryos at 48 hours. Pineal glands are double-stained for Tg(HuC:GFP) (in green) and FRet43 (in red). Anterior is upwards. Scale bar: 16 μm. (D-I) Average numbers of Isl1+ neurons, Tg(AANAT2:GFP)+, lhx3+, FRet43+, HuC/D+ and Isl1+/Pax6+ cells in the pineal gland of control and transgenic Tg(hsp70:bmp2b) embryos heat-shocked at various stages and analyzed at 48 hours. In E and G, Tg(hs:bmp2b) embryos heat-shocked at 21 hours show a relatively mild but statistically significant effect (P=0.03 and P=0.037, respectively). (J-M) Average numbers of Isl1+ neurons, Tg(AANAT2:GFP)+, Fret43+ and HuC/D+ cells in the pineal gland of control and Tg(hs:Gal4); Tg(UAS:caBmpr1a) double-transgenic embryos at 48 hours. Heat shocks were performed at 16 hours. Error bars represent s.d. *P<0.05, **P<0.001, ***P<0.0005 using a t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP fr13Tg; knu3Tg standard conditions Long-pec epiphysis IFL
kca4Tg; knu3Tg; ups5Tg standard conditions Long-pec epiphysis IFL
knu3Tg standard conditions Long-pec epiphysis IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-1 IHC fr13Tg; knu3Tg standard conditions Long-pec epiphysis
IHC kca4Tg; knu3Tg; ups5Tg standard conditions Long-pec epiphysis
IHC knu3Tg standard conditions Long-pec epiphysis
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development