ZFIN ID: ZDB-FIG-110608-15
Quillien et al., 2011 - BMP signaling orchestrates photoreceptor specification in the zebrafish pineal gland in collaboration with Notch. Development (Cambridge, England)   138(11):2293-2302 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Term:
Stage: Long-pec

Fig. 3 BMP activity is sufficient to promote the PhR fate. (A-C) Confocal sections of control, Tg(hsp70:bmp2b) transgenic and Tg(hs:Gal4);Tg(UAS:caBmpr1a) double transgenic embryos at 48 hours. Pineal glands are double-stained for Tg(HuC:GFP) (in green) and FRet43 (in red). Anterior is upwards. Scale bar: 16 μm. (D-I) Average numbers of Isl1+ neurons, Tg(AANAT2:GFP)+, lhx3+, FRet43+, HuC/D+ and Isl1+/Pax6+ cells in the pineal gland of control and transgenic Tg(hsp70:bmp2b) embryos heat-shocked at various stages and analyzed at 48 hours. In E and G, Tg(hs:bmp2b) embryos heat-shocked at 21 hours show a relatively mild but statistically significant effect (P=0.03 and P=0.037, respectively). (J-M) Average numbers of Isl1+ neurons, Tg(AANAT2:GFP)+, Fret43+ and HuC/D+ cells in the pineal gland of control and Tg(hs:Gal4); Tg(UAS:caBmpr1a) double-transgenic embryos at 48 hours. Heat shocks were performed at 16 hours. Error bars represent s.d. *P<0.05, **P<0.001, ***P<0.0005 using a t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP fr13Tg; knu3Tg standard conditions Long-pec epiphysis IFL
kca4Tg; knu3Tg; ups5Tg standard conditions Long-pec epiphysis IFL
knu3Tg standard conditions Long-pec epiphysis IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-1 IHC fr13Tg; knu3Tg standard conditions Long-pec epiphysis
IHC kca4Tg; knu3Tg; ups5Tg standard conditions Long-pec epiphysis
IHC knu3Tg standard conditions Long-pec epiphysis
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development