PUBLICATION
BMP signaling orchestrates photoreceptor specification in the zebrafish pineal gland in collaboration with Notch
- Authors
- Quillien, A., Blanco-Sanchez, B., Halluin, C., Moore, J.C., Lawson, N.D., Blader, P., and Cau, E.
- ID
- ZDB-PUB-110523-30
- Date
- 2011
- Source
- Development (Cambridge, England) 138(11): 2293-2302 (Journal)
- Registered Authors
- Blader, Patrick, Cau, Elise, Halluin, Caroline, Lawson, Nathan, Moore, John
- Keywords
- notch, bone morphogenetic proteins, signal integration
- MeSH Terms
-
- In Situ Hybridization
- Photoreceptor Cells, Vertebrate/cytology*
- Genetic Engineering
- Gene Expression Regulation, Developmental
- Smad5 Protein/metabolism
- Bone Morphogenetic Protein 2/genetics
- Bone Morphogenetic Protein 2/metabolism*
- Signal Transduction*
- Bone Morphogenetic Protein Receptors/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Receptors, Notch/metabolism*
- Cell Differentiation
- Neurons/cytology
- Neurons/metabolism
- Pineal Gland/cytology
- Pineal Gland/embryology*
- Pineal Gland/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Animals
- PubMed
- 21558377 Full text @ Development
Citation
Quillien, A., Blanco-Sanchez, B., Halluin, C., Moore, J.C., Lawson, N.D., Blader, P., and Cau, E. (2011) BMP signaling orchestrates photoreceptor specification in the zebrafish pineal gland in collaboration with Notch. Development (Cambridge, England). 138(11):2293-2302.
Abstract
A variety of signaling pathways have been shown to regulate specification of neuronal subtype identity. However, the mechanisms by which future neurons simultaneously process information from multiple pathways to establish their identity remain poorly understood. The zebrafish pineal gland offers a simple system with which to address questions concerning the integration of signaling pathways during neural specification as it contains only two types of neurons - photoreceptors and projection neurons. We have previously shown that Notch signaling inhibits the projection neuron fate. Here, we show that BMP signaling is both necessary and sufficient to promote the photoreceptor fate. We also demonstrate that crosstalk between BMP and Notch signaling is required for the inhibition of a projection neuron fate in future photoreceptors. In this case, BMP signaling is required as a competence factor for the efficient activation of Notch targets. Our results indicate that both the induction of a photoreceptor fate and the interaction with Notch relies on a canonical BMP/ Smad5 pathway. However, the activation of Notch-dependent transcription does not require a canonical Smad5-DNA interaction. Our results provide new insights into how multiple signaling influences are integrated during cell fate specification in the vertebrate CNS.A variety of signaling pathways have been shown to regulate specification of neuronal subtype identity. However, the mechanisms by which future neurons simultaneously process information from multiple pathways to establish their identity remain poorly understood. The zebrafish pineal gland offers a simple system with which to address questions concerning the integration of signaling pathways during neural specification as it contains only two types of neurons - photoreceptors and projection neurons. We have previously shown that Notch signaling inhibits the projection neuron fate. Here, we show that BMP signaling is both necessary and sufficient to promote the photoreceptor fate. We also demonstrate that crosstalk between BMP and Notch signaling is required for the inhibition of a projection neuron fate in future photoreceptors. In this case, BMP signaling is required as a competence factor for the efficient activation of Notch targets. Our results indicate that both the induction of a photoreceptor fate and the interaction with Notch relies on a canonical BMP/ Smad5 pathway. However, the activation of Notch-dependent transcription does not require a canonical Smad5-DNA interaction. Our results provide new insights into how multiple signaling influences are integrated during cell fate specification in the vertebrate CNS.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping