FIGURE

Fig. 3

ID

ZDB-FIG-110421-5

Publication

Fujimoto et al., 2011 - Identification of a Dopaminergic Enhancer Indicates Complexity in Vertebrate Dopamine Neuron Phenotype Specification

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Fig. 3

Characterization of Tg(otpb.A:egfp)^zc48; confocal images of whole mount embryos double-labeled for GFP and for TH immunohistochemistry, ventral views, anterior to the top. Scale bar is 50 μm. (A) Schematic diagram of TH-positive cell groups in the zebrafish brain at 72 hpf, based on Rink and Wullimann (2002). (B) Confocal z-stack projection of TH immunohistochemistry at 72 hpf in the zebrafish brain. (C) Confocal z-stack projection of GFP immunohistochemistry in Tg(otpb.A:egfp)^zc48. (D–D3) Confocal z-stack projections at different dorsal–ventral levels in the brain of Tg(otpb.A:egfp)^zc48 at 72 hpf, showing co-expression of TH and GFP in diDA neuron groups 4 and 6, but not groups 1 and 2 (arrow); arrowhead points to the NPO neurons. (E–E3) Magnified views of the region boxed in B3, showing extensive overlap of GFP-positive neurons in the diencephalon with TH expression. (F–F3) Expression at 24 hpf. (G–G3) Expression at 48 hpf.

Expression Data

Genes:	EGFP th
Fish:	zc48Tg
Anatomical Term:	diencephalon dopaminergic neuron
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 352(2), Fujimoto, E., Stevenson, T.J., Chien, C.B., and Bonkowsky, J.L., Identification of a Dopaminergic Enhancer Indicates Complexity in Vertebrate Dopamine Neuron Phenotype Specification, 393-404, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.