FIGURE

Fig. 2

ID

ZDB-FIG-110407-12

Publication

Kim et al., 2011 - Zebrafish model of tuberous sclerosis complex reveals cell-autonomous and non-cell-autonomous functions of mutant tuberin

Other Figures

(all 13)

All Figure Page

Back to All Figure Page

Fig. 2

Upregulation of TORC1 activity in various tissues of tsc2^vu242/vu242 mutants. (A?D) Homozygous mutant zebrafish have a deflated swim bladder and markedly enlarged liver at 7 dpf. Livers are outlined (B,D); asterisk indicates swim bladder. (E?P) Sections obtained from wild-type (WT) and tsc2^vu242/vu242 embryos at 7 dpf and stained with anti-phospho-S6 ribosomal protein (Ser235/236) antibody (E?L) or anti-phospho-4E-BP1 (Thr37/46) (M?P). Phospho-S6 staining in the eye (E,H), brain (F,I) and trunk (G,J). Phospho-4E-BP1 staining in the brain (M,O) and trunk (N,P). (Q) Western blot using anti-phospho-S6 ribosomal protein antibody and anti-phospho-4E-BP1 of total protein lysates from 7-dpf wild-type, tsc2^vu242/+ and tsc2^vu242/vu242 larvae. Antibody staining for β-actin was used as a loading control. P, pharynx; K, kidney; I, intestine; L, liver. Scale bars: 100 μm.

Expression Data

Gene:	eif4ebp1
Antibodies:	Ab1-eif4ebp1 Ab1-rps6 Ab2-eif4ebp1 Ab2-rps6
Fish:	AB tsc2^vu242/vu242 (AB) tsc2^vu242/+ (AB)
Anatomical Terms:	brain eye intestine kidney liver pharynx spinal cord whole organism (all 8)
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tsc2^vu242/vu242 (AB)
Observed In:	brain brain protein serine/threonine kinase activity eye protein serine/threonine kinase activity intestine protein serine/threonine kinase activity kidney protein serine/threonine kinase activity liver liver protein serine/threonine kinase activity negative regulation of TOR signaling spinal cord protein serine/threonine kinase activity swim bladder (all 10)
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.