ZFIN ID: ZDB-FIG-110407-12
Kim et al., 2011 - Zebrafish model of tuberous sclerosis complex reveals cell-autonomous and non-cell-autonomous functions of mutant tuberin. Disease models & mechanisms   4(2):255-267 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage: Days 7-13

Fig. 2 Upregulation of TORC1 activity in various tissues of tsc2vu242/vu242 mutants. (A–D) Homozygous mutant zebrafish have a deflated swim bladder and markedly enlarged liver at 7 dpf. Livers are outlined (B,D); asterisk indicates swim bladder. (E–P) Sections obtained from wild-type (WT) and tsc2vu242/vu242 embryos at 7 dpf and stained with anti-phospho-S6 ribosomal protein (Ser235/236) antibody (E–L) or anti-phospho-4E-BP1 (Thr37/46) (M–P). Phospho-S6 staining in the eye (E,H), brain (F,I) and trunk (G,J). Phospho-4E-BP1 staining in the brain (M,O) and trunk (N,P). (Q) Western blot using anti-phospho-S6 ribosomal protein antibody and anti-phospho-4E-BP1 of total protein lysates from 7-dpf wild-type, tsc2vu242/+ and tsc2vu242/vu242 larvae. Antibody staining for β-actin was used as a loading control. P, pharynx; K, kidney; I, intestine; L, liver. Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
eif4ebp1 Ab2-eif4ebp1 AB standard conditions Days 7-13 whole organism WB
Ab2-eif4ebp1 tsc2vu242/+(AB) standard conditions Days 7-13 whole organism WB
Ab1-eif4ebp1 tsc2vu242/vu242(AB) standard conditions Days 7-13 intestine IHC
Ab1-eif4ebp1 Days 7-13 kidney IHC
Ab1-eif4ebp1 Days 7-13 liver IHC
Ab1-eif4ebp1 Days 7-13 pharynx IHC
Ab2-eif4ebp1 Days 7-13 whole organism WB
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-rps6 IHC tsc2vu242/vu242(AB) standard conditions Days 7-13 brain
IHC Days 7-13 eye
IHC Days 7-13 intestine
IHC Days 7-13 liver
IHC Days 7-13 spinal cord
Ab2-rps6 WB AB standard conditions Days 7-13 whole organism
WB tsc2vu242/+(AB) standard conditions Days 7-13 whole organism
WB tsc2vu242/vu242(AB) standard conditions Days 7-13 whole organism
Phenotype Details
Fish Conditions Stage Phenotype
tsc2vu242/vu242(AB) standard conditions Days 7-13 brain morphology, abnormal
Days 7-13 brain protein serine/threonine kinase activity increased occurrence, abnormal
Days 7-13 eye protein serine/threonine kinase activity increased occurrence, abnormal
Days 7-13 intestine protein serine/threonine kinase activity increased occurrence, abnormal
Days 7-13 kidney protein serine/threonine kinase activity increased occurrence, abnormal
Days 7-13 liver increased size, abnormal
Days 7-13 liver protein serine/threonine kinase activity increased occurrence, abnormal
Days 7-13 negative regulation of TOR signaling disrupted, abnormal
Days 7-13 spinal cord protein serine/threonine kinase activity increased occurrence, abnormal
Days 7-13 swim bladder collapsed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.