FIGURE

Fig. 2

ID

ZDB-FIG-110407-12

Publication

Kim et al., 2011 - Zebrafish model of tuberous sclerosis complex reveals cell-autonomous and non-cell-autonomous functions of mutant tuberin

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Fig. 2

Upregulation of TORC1 activity in various tissues of tsc2^vu242/vu242 mutants. (A–D) Homozygous mutant zebrafish have a deflated swim bladder and markedly enlarged liver at 7 dpf. Livers are outlined (B,D); asterisk indicates swim bladder. (E–P) Sections obtained from wild-type (WT) and tsc2^vu242/vu242 embryos at 7 dpf and stained with anti-phospho-S6 ribosomal protein (Ser235/236) antibody (E–L) or anti-phospho-4E-BP1 (Thr37/46) (M–P). Phospho-S6 staining in the eye (E,H), brain (F,I) and trunk (G,J). Phospho-4E-BP1 staining in the brain (M,O) and trunk (N,P). (Q) Western blot using anti-phospho-S6 ribosomal protein antibody and anti-phospho-4E-BP1 of total protein lysates from 7-dpf wild-type, tsc2^vu242/+ and tsc2^vu242/vu242 larvae. Antibody staining for β-actin was used as a loading control. P, pharynx; K, kidney; I, intestine; L, liver. Scale bars: 100 μm.

Expression Data

Gene:	eif4ebp1
Antibodies:	Ab1-eif4ebp1 Ab1-rps6 Ab2-eif4ebp1 Ab2-rps6
Fish:	AB tsc2^vu242/vu242 (AB) tsc2^vu242/+ (AB)
Anatomical Terms:	brain eye intestine kidney liver pharynx spinal cord whole organism
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tsc2^vu242/vu242 (AB)
Observed In:	brain brain protein serine/threonine kinase activity eye protein serine/threonine kinase activity intestine protein serine/threonine kinase activity kidney protein serine/threonine kinase activity liver liver protein serine/threonine kinase activity negative regulation of TOR signaling spinal cord protein serine/threonine kinase activity swim bladder
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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