FIGURE

Fig. S8

ID

ZDB-FIG-110221-52

Publication

Gibert et al., 2011 - BMP Signaling Modulates Hepcidin Expression in Zebrafish Embryos Independent of Hemojuvelin

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Fig. S8

Effect of knockdown of RGM genes at 72 hpf. Whole mount in situ hybridization for hepcidin (A–D) or foxa3 (E–H), dorsolateral views. Compared to uninjected controls (A), knockdown of RGMa (B), RGMb (C), or RGMd (D) failed to inhibit hepcidin expression. E–H. Expression of foxa3 in the liver revealed a significant reduction of liver size in the RGMb and RGMd morphants (G, H). N = 20 embryos per group. I. Quantitative real-time RT-PCR revealed no significant decrease in hepcidin transcript levels relative to liver fatty acid binding protein (LFABP). N = 3 pools of embryos per group. Data shown are means + SE.

Expression Data

Genes:	foxa3 hamp
Fish:	WT WT + MO1-rgma WT + MO1-rgmb WT + MO1-rgmd
Knockdown Reagents:	MO1-rgma MO1-rgmb MO1-rgmd
Anatomical Term:	liver
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-rgmb WT + MO1-rgmd
Knockdown Reagents:	MO1-rgmb MO1-rgmd
Observed In:	liver
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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