FIGURE

Fig. 2

ID

ZDB-FIG-110221-38

Publication

Gibert et al., 2011 - BMP Signaling Modulates Hepcidin Expression in Zebrafish Embryos Independent of Hemojuvelin

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Morpholino knockdown of hjv results in notochord and somite abnormalities.

A–E. Light microscopy of zebrafish embryos at 15 hpf in dorsal view Compared to uninjected embryos (A) or embryos injected with a mismatch control morpholino (E), embryos injected with either single hjv morpholinos (B, C) or a combination of hjv MO1 and hjv MO2 (D) exhibited a distorted notochord (arrows). N = 30 per group. F,G. Whole mount in situ hybridization at 18 hpf with no tail, which stains the notochord, illustrates the bent shape in the hjv MO1 injected morphants. N = 15 per group. H–J. Light microscopy of the tail at 24 hpf lateral view (top) with additional 3.5x enlargement of area labeled in red (below). Somites (arrows) in uninjected (H) and control morpholino-injected embryos (I) appeared V-shaped, while somites appeared U-shaped with decreased anterior-posterior dimension (distance between each pair of arrows) in hjv morphants (J). N = 20 per group.

Expression Data

Gene:	tbxta
Fish:	WT WT + MO1-hjv
Knockdown Reagent:	MO1-hjv
Anatomical Term:	notochord
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-hjv WT + MO1-hjv + MO2-hjv WT + MO2-hjv
Knockdown Reagents:	MO1-hjv MO2-hjv
Observed In:	notochord somite whole organism
Stage Range:	10-13 somites to Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One