|
Fig. 2 Morpholino knockdown of hjv results in notochord and somite abnormalities.
A–E. Light microscopy of zebrafish embryos at 15 hpf in dorsal view Compared to uninjected embryos (A) or embryos injected with a mismatch control morpholino (E), embryos injected with either single hjv morpholinos (B, C) or a combination of hjv MO1 and hjv MO2 (D) exhibited a distorted notochord (arrows). N = 30 per group. F,G. Whole mount in situ hybridization at 18 hpf with no tail, which stains the notochord, illustrates the bent shape in the hjv MO1 injected morphants. N = 15 per group. H–J. Light microscopy of the tail at 24 hpf lateral view (top) with additional 3.5x enlargement of area labeled in red (below). Somites (arrows) in uninjected (H) and control morpholino-injected embryos (I) appeared V-shaped, while somites appeared U-shaped with decreased anterior-posterior dimension (distance between each pair of arrows) in hjv morphants (J). N = 20 per group.