Fig. 3

The moe^rw306 Mutation Affects Neuroepithelial Polarity

(A) Visualization of aPKC (a and b; 18 hpf; e and f; 30 hpf; red), F-actin (c and d; 18 hpf; g and h; 30 hpf; red), ZO-1 (i and j; 30 hpf, red), γ-tubulin (γ-Tub; k and l; 30 hpf, red), GFAP (m and n; 34 hpf, red), fibronectin (FN; o and p; 30 hpf, red), laminin (q and r; 30 hpf, red), and HNK-1 antigen (s and t; 30 hpf, red) in the WT (a, c, e, g, i, k, m, o, q, and s) and moe^rw306 mutant (b, d, f, h, j, l, n, p, r, and t) embryos. The vagus motor neurons are apparent in green (e–l, o–t). Cross-sectional views, dorsal to the top.

(B) Visualization of aPKC (a, c, e, and g; 30 hpf, red) and F-actin (b, d, f, and h; 30 hpf, red) in embryos injected with 5-mis-pair control MO (a and b; 2.5 mg/ml), nok MO (c and d; 2.5 mg/ml), crb2 mRNA (e and f; 500 μg/ml), and aPKCλ MO (g and h; 1.0 mg/ml). The vagus motor neurons are apparent in green. Cross-sectional views, dorsal to the top. (i and j) Dorsal views of isl1:GFP embryos injected with the standard control MO (i; 1.0 mg/ml) and aPKCλ MO (j; 1.0 mg/ml) at 50 hpf (rostral to the top).