Dual Roles of Notch in Regulation of Apically Restricted Mitosis and Apicobasal Polarity of Neuroepithelial Cells
- Authors
- Ohata, S., Aoki, R., Kinoshita, S., Yamaguchi, M., Tsuruoka-Kinoshita, S., Tanaka, H., Wada, H., Watabe, S., Tsuboi, T., Masai, I., and Okamoto, H.
- ID
- ZDB-PUB-110131-12
- Date
- 2011
- Source
- Neuron 69(2): 215-230 (Journal)
- Registered Authors
- Masai, Ichiro, Ohata, Shinya, Okamoto, Hitoshi, Tanaka, Hideomi, Wada, Hironori, Yamaguchi, Masahiro
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Movement/physiology
- Cell Polarity/physiology*
- Embryonic Development/physiology
- Eye Proteins/genetics
- Eye Proteins/metabolism*
- Glycogen Synthase Kinase 3/metabolism
- Mitosis/physiology*
- Motor Neurons/cytology
- Motor Neurons/physiology
- Mutation
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism*
- Neuroepithelial Cells*/cytology
- Neuroepithelial Cells*/physiology
- Proto-Oncogene Proteins c-akt/metabolism
- Receptors, Notch/genetics
- Receptors, Notch/metabolism*
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Stem Cells/cytology
- Stem Cells/physiology
- Zebrafish/anatomy & histology
- Zebrafish/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 21262462 Full text @ Neuron
How the mitosis of neuroepithelial stem cells is restricted to the apical ventricular area remains unclear. In zebrafish, the mosaic eyesrw306 (moe/epb41l5rw306) mutation disrupts the interaction between the putative adaptor protein Moe and the apicobasal polarity regulator Crumbs (Crb), and impairs the maintenance of neuroepithelial apicobasal polarity. While Crb interacts directly with Notch and inhibits its activity, Moe reverses this inhibition. In the moerw306 hindbrain, Notch activity is significantly reduced, and the number of cells that proliferate basally away from the apical area is increased. Surprisingly, activation of Notch in the moerw306 mutant rescues not only the basally localized proliferation but also the aberrant neuroepithelial apicobasal polarity. We present evidence that the Crb⋅Moe complex and Notch play key roles in a positive feedback loop to maintain the apicobasal polarity and the apical-high basal-low gradient of Notch activity in neuroepithelial cells, both of which are essential for their apically restricted mitosis.