ZFIN ID: ZDB-FIG-110214-17
Tittle et al., 2011 - Uhrf1 and Dnmt1 are required for development and maintenance of the zebrafish lens. Developmental Biology   350(1):50-63 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 4

Fig. 5 uhrf1 and dnmt1 are expressed in proliferative regions of the lens and retina at the time of phenotype onset. In situ hybridization of uhrf1 sense (A) and antisense (B–D) and dnmt1 sense (E) and antisense (F–H) riboprobe on wild-type embryos at 4 dpf. No expression was detected in sense controls (A,E). Dorsal (B,F) and lateral (C,G) whole mount images demonstrate that uhrf1 and dnmt1 transcripts are expressed in both the ciliary marginal zone of the retina and in the proliferative germinative zone of the lens epithelium (which resembles a ring around the anterior lens when viewed in whole mount, arrows in C and G). Sectioned images of the lens (D, H) confirm the restriction of uhrf1 and dnmt1 expression to distinct regions of the epithelium (anterior is right, dorsal is up). Scale bars in A–C and E–G are 100 μm, and scale bars in D and H are 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dnmt1 WT standard conditions Day 4 ciliary marginal zone ISH
Day 4 lens epithelium ISH
uhrf1 WT standard conditions Day 4 ciliary marginal zone ISH
Day 4 lens epithelium ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 350(1), Tittle, R.K., Sze, R., Ng, A., Nuckels, R.J., Swartz, M.E., Anderson, R.M., Bosch, J., Stainier, D.Y., Eberhart, J.K., and Gross, J.M., Uhrf1 and Dnmt1 are required for development and maintenance of the zebrafish lens, 50-63, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.