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ZFIN ID: ZDB-FIG-110112-23
Lin et al., 2010 - Loss of Cofilin 1 Disturbs Actin Dynamics, Adhesion between Enveloping and Deep Cell Layers and Cell Movements during Gastrulation in Zebrafish. PLoS One   5(12):e15331 Full text @ PLoS One
Knockdown Reagent:
Anatomical Term:
Stage: Bud
Knockdown Reagent:
Observed In:
Stage: Bud

Fig. 9 Knockdown of cfl1 does not affect involution of mesendoderm.

Embryos injected with 7.5 ng Std MO (A–F), 5.0 ng (G–L) or 7.5 ng cfl1 tMO1 (M–R) were examined at 10 hpf (A, B, G, H, M, N), fixed and subjected to WISH against cstl1b (C–F, I–L, O–R). Representative photographs were taken at a lower (6.3X) or higher (50X) magnification and scale bars are shown at the bottom panel of each column (1 mm for 6.3X and 200 μm for 50X).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb AB/TU standard conditions Bud prechordal plate ISH
AB/TU + MO2-cfl1 standard conditions Bud prechordal plate ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB/TU + MO2-cfl1 standard conditions Bud involution involved in gastrulation with mouth forming second process quality, normal
Bud prechordal plate present, normal
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One