ZFIN ID: ZDB-FIG-110112-21
Lin et al., 2010 - Loss of Cofilin 1 Disturbs Actin Dynamics, Adhesion between Enveloping and Deep Cell Layers and Cell Movements during Gastrulation in Zebrafish. PLoS One   5(12):e15331 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Shield to 75%-epiboly

Fig. 7 Live cell imaging reveals loose cell-cell interactions between the DEL and EVL in cfl1 morphants.

Embryos injected with designated MOs and membrane-bound green fluorescent protein (GFP) mRNA, were dechorionated, immobilized, and examined by confocal microscopy for 10-min recordings at 10-s intervals per frame. The snapshots of representative embryos from the StdMO- (left column) or cfl1MO-injected embryos (right column) are shown. The recording times in minutes are denoted in the lower left corner of the left column. While StdMO-treated embryos showed continuous tight attachments between the DEL and EVL, DEL cells in cfl1 morphants did not form a tight connection with the EVL, which was further evidenced by the more-rounded cell shapes. Arrowheads indicate spaces between the EVL and DEL. A single-cell-thick EVL is to the right; yolk cells are toward the left; the animal pore is on the top; and the vegetal pore is at the bottom.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB/TU + MO2-cfl1 standard conditions Shield - 75%-epiboly cell migration involved in gastrulation process quality, abnormal
Shield - 75%-epiboly cell-cell adhesion involved in gastrulation decreased occurrence, abnormal
Shield - 75%-epiboly DEL circular, abnormal
Shield - 75%-epiboly DEL detached from EVL, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One