Live cell imaging reveals loose cell-cell interactions between the DEL and EVL in cfl1 morphants.
Embryos injected with designated MOs and membrane-bound green fluorescent protein (GFP) mRNA, were dechorionated, immobilized, and examined by confocal microscopy for 10-min recordings at 10-s intervals per frame. The snapshots of representative embryos from the StdMO- (left column) or cfl1MO-injected embryos (right column) are shown. The recording times in minutes are denoted in the lower left corner of the left column. While StdMO-treated embryos showed continuous tight attachments between the DEL and EVL, DEL cells in cfl1 morphants did not form a tight connection with the EVL, which was further evidenced by the more-rounded cell shapes. Arrowheads indicate spaces between the EVL and DEL. A single-cell-thick EVL is to the right; yolk cells are toward the left; the animal pore is on the top; and the vegetal pore is at the bottom.