Morpholino

MO1-shha

ID

ZDB-MRPHLNO-050204-6

Name

MO1-shha

Previous Names

MO(T)-shh
MO1-shh (1)
shh-MO (2)

Target

shha (1)

Sequence

5' - CAGCACTCTCGTCAAAAGCCGCATT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

A translation blocking morpholino against shh. Embryos have a normal head and u-shaped somites. The myoseptum is absent. The finbuds are reduced compared with wild type embryos.

Genome Resources

None

Target Location

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO1-shha

No data available

Phenotype

Phenotype resulting from MO1-shha

Phenotype	Fish	Figures
brain glis3 expression absent, abnormal	AB + MO1-shha	Fig. 5 from Rurale et al., 2019
endoderm glis3 expression absent, abnormal	AB + MO1-shha	Fig. 5 from Rurale et al., 2019
eye pax6a expression decreased amount, abnormal	AB + MO1-shha	Fig. 2 from Zhang et al., 2017
forebrain pax6a expression decreased amount, abnormal	AB + MO1-shha	Fig. 2 from Zhang et al., 2017
hindbrain pax6a expression decreased amount, abnormal	AB + MO1-shha	Fig. 2 from Zhang et al., 2017
midbrain hindbrain boundary morphology, abnormal	AB + MO1-shha	Fig. 5 from Zhang et al., 2017
odontogenesis arrested, abnormal	WT + MO1-shha	Fig. 3 from Jackman et al., 2010
pancreas primordium Ab8-ins labeling decreased amount, abnormal	AB + MO1-shha	Fig. 5 from Yang et al., 2021
pancreas primordium ab2-isl labeling decreased amount, abnormal	AB + MO1-shha	Fig. 5 from Yang et al., 2021
slow muscle cell nucleus decreased amount, abnormal	WT + MO1-shha	Fig. 6 from Philipp et al., 2008
somite muscle pioneer decreased amount, abnormal	WT + MO1-shha	Fig. 6 from Philipp et al., 2008
whole organism lacks all parts of type ceratobranchial 5 tooth, abnormal	WT + MO1-shha	Fig. 3 from Jackman et al., 2010

Phenotype of all Fish created by or utilizing MO1-shha

Phenotype	Fish	Conditions	Figures
pancreas primordium ab2-isl labeling decreased amount, abnormal	AB + MO1-shha	standard conditions	Fig. 5 from Yang et al., 2021
midbrain hindbrain boundary present, ameliorated	AB + MO1-shha	chemical treatment: ethanol, chemical treatment: retinoic acid	Fig. 1 from Zhang et al., 2015
swimming behavior process quality, abnormal	AB + MO1-shha	chemical treatment by environment: ethanol	Fig. 4, Fig. 5 from Burton et al., 2017
swimming behavior disrupted, abnormal	AB + MO1-shha	chemical treatment by environment: ethanol	Fig. 7, Fig. 8 from Boa-Amponsem et al., 2020
midbrain hindbrain boundary absent, abnormal	AB + MO1-shha	chemical treatment: ethanol	Fig. 1 from Zhang et al., 2015
hindbrain pax6a expression decreased amount, abnormal	AB + MO1-shha	standard conditions	Fig. 2 from Zhang et al., 2017
pancreas primordium Ab8-ins labeling decreased amount, abnormal	AB + MO1-shha	standard conditions	Fig. 5 from Yang et al., 2021
eye decreased size, abnormal	AB + MO1-shha	chemical treatment by environment: ethanol	Fig. 2, Fig. 3 from Boa-Amponsem et al., 2020
endoderm glis3 expression absent, abnormal	AB + MO1-shha	control	Fig. 5 from Rurale et al., 2019
forebrain pax6a expression decreased amount, abnormal	AB + MO1-shha	standard conditions	Fig. 2 from Zhang et al., 2017
eye decreased size, abnormal	AB + MO1-shha	chemical treatment: ethanol	Fig. 6 from Zhang et al., 2015
midbrain hindbrain boundary morphology, abnormal	AB + MO1-shha	standard conditions	Fig. 5 from Zhang et al., 2017
eye decreased size, abnormal	AB + MO1-shha	chemical treatment: ethanol, chemical treatment: retinoic acid	Fig. 6 from Zhang et al., 2015
brain glis3 expression absent, abnormal	AB + MO1-shha	control	Fig. 5 from Rurale et al., 2019
eye pax6a expression decreased amount, abnormal	AB + MO1-shha	standard conditions	Fig. 2 from Zhang et al., 2017
midbrain hindbrain boundary malformed, abnormal	AB + MO1-shha	chemical treatment by environment: ethanol	Fig. 2 from Burton et al., 2017
eye decreased size, abnormal	AB + MO1-shha	chemical treatment by environment: ethanol	Fig. 3 from Burton et al., 2017
slow muscle cell nucleus decreased amount, abnormal	WT + MO1-shha	standard conditions	Fig. 6 from Philipp et al., 2008
whole organism lacks all parts of type ceratobranchial 5 tooth, abnormal	WT + MO1-shha	standard conditions	Fig. 3 from Jackman et al., 2010
odontogenesis arrested, abnormal	WT + MO1-shha	standard conditions	Fig. 3 from Jackman et al., 2010
somite muscle pioneer decreased amount, abnormal	WT + MO1-shha	standard conditions	Fig. 6 from Philipp et al., 2008
midbrain-hindbrain boundary development process quality, abnormal	WT + MO1-shha	chemical treatment: ethanol	Fig. 10, Fig. S1 from Zhang et al., 2014
eye decreased size, abnormal	WT + MO1-shha	chemical treatment: ethanol	Fig. S1 from Zhang et al., 2014
pancreas primordium Ab8-ins labeling amount, ameliorated	cq109Tg/cq109Tg + MO1-shha	standard conditions	Fig. 5 from Yang et al., 2021
pancreas primordium ab2-isl labeling amount, ameliorated	cq109Tg/cq109Tg + MO1-shha	standard conditions	Fig. 5 from Yang et al., 2021
midbrain hindbrain boundary absent, abnormal	AB + MO1-aldh1a3 + MO1-shha	control	Fig. 1 from Zhang et al., 2015
midbrain hindbrain boundary present, ameliorated	AB + MO1-aldh1a3 + MO1-shha	chemical treatment: retinoic acid	Fig. 1 from Zhang et al., 2015
pancreas primordium Ab8-ins labeling amount, ameliorated	AB + MO1-shha + MO2-gmnn	standard conditions	Fig. 5 from Yang et al., 2021
pancreas primordium ab2-isl labeling amount, ameliorated	AB + MO1-shha + MO2-gmnn	standard conditions	Fig. 5 from Yang et al., 2021
slow muscle cell nucleus decreased amount, abnormal	WT + MO1-grk3 + MO1-shha	standard conditions	Fig. 6 from Philipp et al., 2008
somite muscle pioneer decreased amount, abnormal	WT + MO1-grk3 + MO1-shha	standard conditions	Fig. 6 from Philipp et al., 2008
odontogenesis delayed, abnormal	WT + MO1-shha + MO2-shhb	standard conditions	Fig. 3 from Jackman et al., 2010
tooth placode position, abnormal	WT + MO1-shha + MO2-shhb	standard conditions	Fig. 3 from Jackman et al., 2010

Citations