ZFIN ID: ZDB-FIG-101206-6
Hilario et al., 2010 - Collagen XIXa1 is crucial for motor axon navigation at intermediate targets. Development (Cambridge, England)   137(24):4261-4269 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 3 ColXIX knock down phenocopies the stumpy mutation. (A) RT-PCR shows inclusion of a 109 bp fragment in splice-blocking colXIX MO injected (MO) compared to wild-type (wt) embryos. (B-D) CaP axon phenotypes as visualized using znp-1 antibody in (B) uninjected wild-type embryos, (C) wild-type embryos injected with 9 ng colXIX MO and (D) stumpyb393-/- mutant embryos. White dashed line indicates the horizontal myoseptum. Scale bar: 70 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col19a1 AB standard conditions Prim-5 whole organism RTPCR
AB + MO2-col19a1 standard conditions Prim-5 whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
znp-1 IHC AB standard conditions Prim-5 CaP motoneuron axon
IHC AB + MO2-col19a1 standard conditions Prim-5 CaP motoneuron axon
IHC col19a1b393/b393(AB) standard conditions Prim-5 CaP motoneuron axon
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-col19a1 standard conditions Prim-5 axon extension involved in axon guidance arrested, abnormal
Prim-5 CaP motoneuron axon decreased length, abnormal
col19a1b393/b393(AB) standard conditions Prim-5 axon extension involved in axon guidance arrested, abnormal
Prim-5 CaP motoneuron axon decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development