FIGURE

Fig. 6

ID

ZDB-FIG-101206-48

Publication

Choy et al., 2010 - A cascade of irx1a and irx2a controls shh expression during retinogenesis

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Fig. 6

irx2a acts as downstream of irx1a and ath5 in controlling retinogenesis. The rescue activity of irx2a mRNA in irx1a MO2 morphants was indicated by retinal cell markers of 72 hpf embryos. Differentiation of the GCL (A–C), INL (C), and ONL (D) was disrupted in irx1a morphant retinas. By co-injecting the irx2a mRNA with irx1a MO2, the retinogenesis defects in GCL and INL were rescued. Immunostaining of eye makers was observed in the co-injected embryos (E–G). However, the differentiation defect in the ONL of irx1a morphant could not be rescued by irx2a mRNA (H). Immunostaining revealed retinal defects in irx1a morphants co-injected with irx4a mRNA (I–L) similar to those observed in irx1a morphants (A–D). M: The rescue activity of irx2a in irx1a morphants was dose-dependent whereas the ath5 morphant phenotype could not be rescued by irx2a and/or irx1a. N: The sub-lethal dose of irx1a or irx2a morpholino MO2 could not induce eye defects whereas co-injection of the same dose of irx1a and irx2a morpholinos induced a significant eye phenotype. Similar results were obtained in experiments with co-injection of a sub-lethal dose of irx2a and ath5 morpholinos.

Expression Data

Antibodies:	Ab1-elavl zn-12 zpr-1
Fish:	WT + MO4-irx1a
Knockdown Reagent:	MO4-irx1a
Anatomical Terms:	retinal cone cell retinal ganglion cell layer retinal inner nuclear layer
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-atoh7 WT + MO4-irx1a WT + MO2-atoh7 + MO2-irx2a WT + MO2-irx2a + MO4-irx1a
Knockdown Reagents:	MO2-atoh7 MO2-irx2a MO4-irx1a
Observed In:	eye neural retina development retinal ganglion cell layer retinal inner nuclear layer
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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