ZFIN ID: ZDB-FIG-101122-53
Ragvin et al., 2010 - Long-range gene regulation links genomic type 2 diabetes and obesity risk regions to HHEX, SOX4, and IRX3. Proceedings of the National Academy of Sciences of the United States of America   107(2):775-780 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 3

Pancreatic buds of 48-hpf zebrafish embryos showing a reduction in the number of insulin-expressing cells and an increase of ghrelin-expressing cells in irx3a morphants. In situ hybridization with insulin (red) and ghrelin (blue) probes on control (A) and irx3a morphant embryos (B). Statistical support for the observation is given in Table 3. (C and D) irx3a morphants display a reduction in pancreatic α-cells. In situ hybridization with glucagon (red in C and D), somatostatin (red in E and F), and ghrelin (blue in CF) probes on control (C and E) and irx3a morphant embryos (D and F). showing reduction in the number of α-cells (D) and increase of ghrelin-expressing cells (D and F) in the pancreatic bud of 48-hpf irx3a morphant embryos. The number of δ-cells is unaffected. Embryos are in ventral view. Statistical support for the observation is given in Table 4.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gcga WT control Long-pec pancreatic bud pancreatic A cell ISH
WT + MO1-irx3a + MO2-irx3a standard conditions Long-pec pancreatic bud pancreatic A cell ISH
ghrl WT control Long-pec pancreatic bud ghrelin secreting cell ISH
WT + MO1-irx3a + MO2-irx3a standard conditions Long-pec pancreatic bud ghrelin secreting cell ISH
ins WT control Long-pec pancreatic bud insulin secreting cell ISH
WT + MO1-irx3a + MO2-irx3a standard conditions Long-pec pancreatic bud insulin secreting cell ISH
sst2 WT control Long-pec pancreatic bud somatostatin secreting cell ISH
WT + MO1-irx3a + MO2-irx3a standard conditions Long-pec pancreatic bud somatostatin secreting cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-irx3a + MO2-irx3a standard conditions Long-pec pancreatic bud has extra parts of type pancreatic epsilon cell, abnormal
Long-pec pancreatic bud has fewer parts of type pancreatic A cell, abnormal
Long-pec pancreatic bud has fewer parts of type pancreatic B cell, abnormal
Long-pec pancreatic bud has normal numbers of parts of type D cell, normal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA