ZFIN ID: ZDB-FIG-101108-5
Hellman et al., 2010 - The zebrafish foxj1a transcription factor regulates cilia function in response to injury and epithelial stretch. Proceedings of the National Academy of Sciences of the United States of America   107(43):18499-18504 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Long-pec

Fig. S1 foxj1a and foxj1b expression in ciliated cell types at different developmental stages. (A and B) foxj1a is uniformly expressed in precursors to the kidney and spinal cord at the 12-somite stage, as well as in Kupffer’s vesicle (arrowhead). (C) At 24, hpf foxj1a is expressed throughout the spinal cord (arrow) and in all cells of the paired pronephric ducts (arrowhead). (D) By 48 hpf, foxj1a is down-regulated in singly ciliated cells of the pronephros but is maintained in a “salt-and-pepper” pattern in tubule cells, consistent with expression in multiciliated cells. (E) At 48 hpf, expression is also observed in the olfactory placode (arrowhead) and brain ventricular system (arrow). (F) Spinal cord is positive for foxj1a at 48 hpf. (G and H) foxj1b is expressed in developing otic placodes (arrowheads) at the 12-somite stage, and (I) in pronephric multiciliated cells (arrowhead) at 48 hpf. Note that foxj1b expression is seen only at the most distal tip of the spinal cord (arrow).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxj1a WT standard conditions 10-13 somites intermediate mesoderm ISH
10-13 somites Kupffer's vesicle ISH
10-13 somites neural tube ISH
Prim-5 pronephric duct ISH
Prim-5 spinal cord ISH
Long-pec peripheral olfactory organ ISH
Long-pec pronephros multi-ciliated epithelial cell ISH
Long-pec spinal cord ISH
Long-pec ventricular system epithelium ISH
foxj1b WT standard conditions 10-13 somites otic placode ISH
Long-pec pronephros multi-ciliated epithelial cell ISH
Long-pec spinal cord posterior side ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA