FIGURE

Fig. 4

ID

ZDB-FIG-101108-4

Publication

Hellman et al., 2010 - The zebrafish foxj1a transcription factor regulates cilia function in response to injury and epithelial stretch

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Fig. 4

foxj1a target genes are up-regulated in response to tubule obstruction. Control expression of foxj1a target genes efhc1 (A), tektin-1 (D), and dnahc9 (G) at 56 hpf is up-regulated by distal pronephric obstruction (B, E, and H). foxj1a knockdown prevented up-regulation in response to tubule obstruction (C, F, and I). Quantitative real-time RT-PCR (J) confirmed up-regulation of foxj1a target gene expression in obstructed tubules (dnahc9 13.3-fold; efch1 3.4-fold, and tektin1 2.6-fold) and inhibition of induction in foxj1a-deficient obstructed tubules. Error bars in J represent range of minimum and maximum fold foxj1a induction relative to control embryos (comparative threshold cycle method) (59).

Expression Data

Genes:	dnah9 efhc1 tekt1
Fish:	WT WT + MO5-foxj1a + MO6-foxj1a
Condition:	physical alteration: anatomical structure
Knockdown Reagents:	MO5-foxj1a MO6-foxj1a
Anatomical Terms:	pronephric tubule multi-ciliated epithelial cell pronephric tubule single ciliated epithelial cell whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO5-foxj1a + MO6-foxj1a
Condition:	physical alteration: anatomical structure
Knockdown Reagents:	MO5-foxj1a MO6-foxj1a
Observed In:	renal tubule development response to wounding
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA