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ZFIN ID: ZDB-FIG-101108-4
Hellman et al., 2010 - The zebrafish foxj1a transcription factor regulates cilia function in response to injury and epithelial stretch. Proceedings of the National Academy of Sciences of the United States of America   107(43):18499-18504 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 4

foxj1a target genes are up-regulated in response to tubule obstruction. Control expression of foxj1a target genes efhc1 (A), tektin-1 (D), and dnahc9 (G) at 56 hpf is up-regulated by distal pronephric obstruction (B, E, and H). foxj1a knockdown prevented up-regulation in response to tubule obstruction (C, F, and I). Quantitative real-time RT-PCR (J) confirmed up-regulation of foxj1a target gene expression in obstructed tubules (dnahc9 13.3-fold; efch1 3.4-fold, and tektin1 2.6-fold) and inhibition of induction in foxj1a-deficient obstructed tubules. Error bars in J represent range of minimum and maximum fold foxj1a induction relative to control embryos (comparative threshold cycle method) (59).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dnah9 WT control Long-pec pronephric tubule multi-ciliated epithelial cell ISH
Long-pec whole organism RTPCR
WT physical alteration: anatomical structure Long-pec pronephric tubule multi-ciliated epithelial cell ISH
Long-pec pronephric tubule single ciliated epithelial cell ISH
Long-pec whole organism RTPCR
WT + MO5-foxj1a + MO6-foxj1a physical alteration: anatomical structure Long-pec pronephric tubule multi-ciliated epithelial cell ISH
Long-pec whole organism RTPCR
efhc1 WT control Long-pec pronephric tubule multi-ciliated epithelial cell ISH
Long-pec whole organism RTPCR
WT physical alteration: anatomical structure Long-pec pronephric tubule multi-ciliated epithelial cell ISH
Long-pec pronephric tubule single ciliated epithelial cell ISH
Long-pec whole organism RTPCR
WT + MO5-foxj1a + MO6-foxj1a physical alteration: anatomical structure Long-pec pronephric tubule multi-ciliated epithelial cell ISH
Long-pec whole organism RTPCR
tekt1 WT control Long-pec pronephric tubule multi-ciliated epithelial cell ISH
Long-pec whole organism RTPCR
WT physical alteration: anatomical structure Long-pec pronephric tubule multi-ciliated epithelial cell ISH
Long-pec pronephric tubule single ciliated epithelial cell ISH
Long-pec whole organism RTPCR
WT + MO5-foxj1a + MO6-foxj1a physical alteration: anatomical structure Long-pec pronephric tubule multi-ciliated epithelial cell ISH
Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT physical alteration: anatomical structure Long-pec renal tubule development process quality, abnormal
WT + MO5-foxj1a + MO6-foxj1a standard conditions Long-pec response to wounding decreased process quality, abnormal
WT + MO5-foxj1a + MO6-foxj1a physical alteration: anatomical structure Long-pec renal tubule development process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA