FIGURE

Fig. 2

ID
ZDB-FIG-100913-1
Publication
McDearmid et al., 2006 - Glycine receptors regulate interneuron differentiation during spinal network development
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Synaptic activity is reduced in α2 GlyR knockdown fish. Spinal motoneuron responses evoked by brief touches in wild-type zebrafish (A; n = 5/5) and GlyR α2 knockdown zebrafish (B; α2 k/d; n = 9/9) at 3 days in development. Arrowheads indicate onset of touch. (C and D) Glycinergic mPSCs (isolated with 1 μM TTX, 10 μM bicuculline, and 1 mM kynurenic acid) in spinal motoneurons voltage-clamped at -60 mV in wild-type zebrafish (C; n = 11) and GlyR α2 knockdown zebrafish (D; α2 k/d; n = 10). Glutamatergic mPSCs (isolated with 1 μM TTX, 10 μM bicuculline, and 1 μM strychnine) in spinal motoneurons voltage-clamped at -65 mV in wild-type zebrafish (E; n = 12) and 3-day GlyR α2 knockdown zebrafish (F; n = 10). CF Left display 500 overlaps of consecutive 200-ms traces of activity. CF Right display 25 averaged mPSCs for each condition. (G and H) Peak conductance, rise time, half-width, and frequency of GlyR mPSCs (G) and glutamatergic mPSCs (H) in wild-type zebrafish and α2 knockdown zebrafish (α2 k/d). -, P < 0.01.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA