|
Fig. 2
Synaptic activity is reduced in α2 GlyR knockdown fish. Spinal motoneuron responses evoked by brief touches in wild-type zebrafish (A; n = 5/5) and GlyR α2 knockdown zebrafish (B; α2 k/d; n = 9/9) at 3 days in development. Arrowheads indicate onset of touch. (C and D) Glycinergic mPSCs (isolated with 1 μM TTX, 10 μM bicuculline, and 1 mM kynurenic acid) in spinal motoneurons voltage-clamped at -60 mV in wild-type zebrafish (C; n = 11) and GlyR α2 knockdown zebrafish (D; α2 k/d; n = 10). Glutamatergic mPSCs (isolated with 1 μM TTX, 10 μM bicuculline, and 1 μM strychnine) in spinal motoneurons voltage-clamped at -65 mV in wild-type zebrafish (E; n = 12) and 3-day GlyR α2 knockdown zebrafish (F; n = 10). C–F Left display 500 overlaps of consecutive 200-ms traces of activity. C–F Right display 25 averaged mPSCs for each condition. (G and H) Peak conductance, rise time, half-width, and frequency of GlyR mPSCs (G) and glutamatergic mPSCs (H) in wild-type zebrafish and α2 knockdown zebrafish (α2 k/d). -, P < 0.01.