FIGURE

Fig. 1

ID

ZDB-FIG-100831-4

Publication

Chi et al., 2010 - Cardiac conduction is required to preserve cardiac chamber morphology

Other Figures

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Fig. 1

dococ^s226 Mutants exhibit pericardial edema and cardiac failure due to dysynchronous contraction of the ventricle. (A) Brightfield micrographs of 48 hpf WT (WT) and dco^s226 mutant (dco ^-/-) embryos. Black arrow points to pericardial edema. (B and C) Confocal micrographs of 60 hpf Tg(flk1:EGFP)^s843 (B) WT and (C) dco^s226 mutant hearts (endothelium green) stained with rhodamine phalloidin (myocardium red). dco Mutant ventricles fail to contract coordinately and exhibit disorganized chamber walls. White arrowheads point to atrioventricular canal; yellow arrows point to outflow tract; white asterisk denotes aberrant ventricular wall contraction. (D) Genetic map of the dco region. Numbers below SSLP markers indicate recombination events out of 922 diploid embryos examined. (E) Sequencing of cx46 cDNA revealed a C-to-T change at base pair 494 in the s226 mutant allele, resulting in a Thr-to-Ile substitution at residue 165. (F) Schematic diagram illustrating modular structure of Cx46. A connexon consists of four transmembrane domains (M1–M4). The M3 domain is the major pore-lining domain. Red asterisk marks site affected by s226 mutation.

Expression Data

Gene:	EGFP
Fish:	s843Tg gja3^s226/s226; s843Tg
Anatomical Term:	heart vascular endothelium
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gja3^s226/s226 gja3^s226/s226; s843Tg
Observed In:	cardiac ventricle heart heart contraction
Stage Range:	Long-pec to Pec-fin

Phenotype Detail

Acknowledgments

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