FIGURE

Fig. S3

ID

ZDB-FIG-100831-3

Publication

Chi et al., 2010 - Cardiac conduction is required to preserve cardiac chamber morphology

Other Figures

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Fig. S3

cx46 Expression and morpholino knockdown confirms that cx46 is the affected gene in dco mutants. (A–D) Whole-mount RNA in situ hybridization reveals cx46 expression in the heart and lens. Expression of cx46 at (A) 36, (B) 42, (C) 48, and (D) 72 hpf. A, atrium; V, ventricle. (E) cx46 and β-Actin control RT-PCR from FACS-sorted myocardial and endocardial cells at 36, 48, and 72 hpf. (F) Morpholino knockdown of cx46 recapitulates dco cardiac phenotype. Of WT embryos injected with an ATG cx46 morpholino, 88% are indistinguishable from dco mutant embryos and display severe impairment of cardiac conduction (n = 125/142).

Expression Data

Gene:	gja3
Fish:	WT
Anatomical Terms:	atrioventricular canal cardiac ventricle heart heart tube myocardium
Stage Range:	Prim-25 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-gja3
Knockdown Reagent:	MO1-gja3
Observed In:	cardiac conduction system development heart contraction
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA