ZFIN Image: Chi et al., 2010, Fig. S3

Image

Figure Caption

Fig. S3 cx46 Expression and morpholino knockdown confirms that cx46 is the affected gene in dco mutants. (A–D) Whole-mount RNA in situ hybridization reveals cx46 expression in the heart and lens. Expression of cx46 at (A) 36, (B) 42, (C) 48, and (D) 72 hpf. A, atrium; V, ventricle. (E) cx46 and β-Actin control RT-PCR from FACS-sorted myocardial and endocardial cells at 36, 48, and 72 hpf. (F) Morpholino knockdown of cx46 recapitulates dco cardiac phenotype. Of WT embryos injected with an ATG cx46 morpholino, 88% are indistinguishable from dco mutant embryos and display severe impairment of cardiac conduction (n = 125/142).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA