FIGURE

Fig. 8

ID

ZDB-FIG-100806-63

Publication

Yang et al., 2010 - Regulatory interactions specifying Kolmer-Agduhr interneurons

Other Figures

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Fig. 8

gata2 and gata3 expression in the lateral floor plate requires Nkx2 genes but not tal2. (A,B) Zebrafish embryos injected with a mix of mismatch morpholinos (A) or morpholinos directed against nkx2.2a, nkx2.2b and nkx2.9 mRNA (B). gata3-expressing cells are not detectable in the lateral floor plate (arrow) in triple morphants, whereas dorsally located gata3-expressing cells (arrowhead) are present. (C,D) Embryos injected with a mismatch control morpholino (C) or a morpholino directed against tal2 mRNA (D). tal2 knockdown does not affect gata3 expression, indicating that tal2 does not regulate gata3. (E,F) Embryos were injected with a mismatch morpholino (E) or a morpholino directed against tal2 mRNA (F). gata2 expression is not abolished by knockdown of tal2 expression. Embryos were 24 hpf. Anterior left, dorsal up. Scale bar: 25 μm.

Expression Data

Genes:	gata2a gata3
Fish:	WT WT + MO1-tal2 WT + MO1-nkx2.9 + MO2-nkx2.2b + MO4-nkx2.2a
Knockdown Reagents:	MO1-nkx2.9 MO1-tal2 MO2-nkx2.2b MO4-nkx2.2a
Anatomical Terms:	lateral floor plate Kolmer-Agduhr neuron spinal cord Kolmer-Agduhr neuron VeLD
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-nkx2.9 + MO2-nkx2.2b + MO4-nkx2.2a
Knockdown Reagents:	MO1-nkx2.9 MO2-nkx2.2b MO4-nkx2.2a
Observed In:	lateral floor plate Kolmer-Agduhr neuron ventral spinal cord interneuron differentiation
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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