FIGURE

Fig. 6

ID

ZDB-FIG-100707-21

Publication

Wiens et al., 2010 - Platelet-derived growth factor receptor Beta is critical for zebrafish intersegmental vessel formation

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Fig. 6

A dominant-negative PDGFRβ blocked ISV angiogenesis in zebrafish embryos.

A heat shock-inducible dominant negative form of PDGFR? was created by substituting the intracellular kinase domains of PDGFR? with YFP (A, dnPDGFR?-YFP). Heat shock at 10 hpf led to dnPDGFR?-YFP expression within 7 hours after heat shock with no effect on overall morphology (B). Heat shock at 20 hpf and analysis at 48 hpf indicated an increase in ISV defects in heat shocked embryos positive for dnPDGFR?-YFP versus heat shocked embryos negative for dnPDGFR?-YFP (C and E, n = 23). Embryos heat shocked at 20 hpf that were positive for dnPDGFR?-YFP at 48 hpf showed no overall morphology defects as compared to embryos heat shocked at 20 hpf negative for dnPDGFR?-YFP (D). Arrowheads indicate complete ISVs. Asterisks indicate angiogenic sprouts. ** indicates p<0.01. All data represent the mean +/- standard error.

Expression Data

Genes:	EGFP YFP
Fish:	kca4Tg; sr1Tg; y1Tg
Condition:	heat shock
Anatomical Terms:	axial vasculature notochord
Stage Range:	14-19 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kca4Tg; sr1Tg; y1Tg
Condition:	heat shock
Observed In:	dorsal longitudinal anastomotic vessel intersegmental vessel
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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