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PDGFR?2 activity was required for ISV angiogenesis in zebrafish embryos. fli1a:eGFP or kdrl:GFP embryos were injected with control morpholino oligonucleotide (MO) or pdgfr?2 MO at the one-cell stage and imaged at 24 hpf (A), 30 hpf and 48 hpf (E). Light images of whole embryos are shown for 24 hpf (B) and 48 hpf (G). Blocking of splicing was confirmed using RT-PCR (D). pdgfr?2 MO-injected embryos had significantly more ISV defects than control MO-injected embryos at 24 hpf (A and C), 30 hpf and 48 hpf (E and F). Angiography at 72 hpf showed less circulation through ISVs in pdgfr?2 MO-injected embryos (H). The ISV defect seen in pdgfr?2 MO-injected embryos was partially rescued when pdgfr?2 mRNA was coinjected with pdgfr?2 MO (I and J). Arrowheads indicate complete ISVs. Asterisks indicate angiogenic sprouts. *** indicates p<0.001, ** indicates p<0.01. All data represent the mean +/- standard error.
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