FIGURE

Fig. 2

ID

ZDB-FIG-100707-2

Publication

Valentin et al., 2007 - The chemokine SDF1a coordinates tissue migration through the spatially restricted activation of Cxcr7 and Cxcr4b

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Fig. 2

The Zebrafish Homolog of Cxcr7 Rdc1 Is Essential for Primordium Migration

(A) Fluorescent in situ hybridization of Cxcr7 and Cxcr4b in the wild-type CldnB::lynGFP background at 36 hr postfertilization. The middle panel shows the restricted expression pattern of Cxcr7 at the back of the primordium and in the deposited chain cells. The lower panel reveals Cxcr4b expression in the whole primordium.

(B and C) Quantification of primordium migration (as in Figure 1C) in wild-type embryos, wild-type embryos injected with control morpholino (Control Cxcr7Mo, 1 mM), or two morpholinos against Cxcr7 (Cxcr7Mo1, Cxcr7Mo2, 1 mM), and cxcr4b embryos injected with Cxcr7Mo1. Wild-type and control morpholino-injected embryos migrate at a similar rate (blue and yellow bars). Primordia treated with the two morpholinos against Cxcr7 (red and purple bars) stop preferentially in part 2; only a few reach parts 3 and 4 or halt already in sector 1. Injection of Cxcr7Mo1 in cxcr4b embryos recapitulates the sdf1a mutant phenotype (green bars, compare Figure 1D).

Expression Data

Genes:	ackr3b cxcr4b
Fish:	zf106Tg
Anatomical Terms:	interneuromast cell lateral line primordium neuromast
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf106Tg + MO5-ackr3b zf106Tg + MO6-ackr3b cxcr4b^{t26035/t26035}; zf106Tg + MO6-ackr3b
Knockdown Reagents:	MO5-ackr3b MO6-ackr3b
Observed In:	posterior lateral line neuromast primordium migration
Stage:	High-pec

Phenotype Detail

Acknowledgments

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