ZFIN ID: ZDB-FIG-100628-36
Vaccari et al., 2010 - prep1.2 and aldh1a2 participate to a positive loop required for branchial arches development in zebrafish. Developmental Biology   343(1-2):94-103 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 10-13 somites to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-25 to Long-pec

Fig. 3 Characterization of prep1.2 function. A–J: Expression pattern of pre-migratory and migratory NCC markers. A, B: prep1.2 knock-down does not affect foxd3 expression in 10 somite stage embryos (compare A and B); C–F: dlx2a expression in NCC is unaffected in prep1.2 morphants analysed at 10 and 20 somite stages; G and H: at 24 hpf expression of dlx2a is strongly downregulated in the third stream of cephalic NCC of MO1-prep1.2 injected embryos; I and J: similar results are obtained in 48 hpf embryos: in prep1.2 morphant embryos dlx2a expression disappears in posterior branchial arches (J). K–P: Failure of the posterior pharyngeal endoderm segmentation in prep1.2 morphant embryos. K–N: Analysis of pharyngeal pouches development in the glu:GFP transgenic line that express GFP ectopically in the pharyngeal endoderm and in embryos stained with Zn5 antibodies. L: in MO1-prep1.2 injected embryos analyzed at 36 hpf, the more posterior pouches (ep3–ep5) do not develop (compare with control in K). Identical results were obtained when using Zn5 antibodies to stain for the pharyngeal endoderm (compare N with control in M, see also Supplemental Fig. 5). O and P; lack of fgf3 expression in the pharyngeal endoderm of prep1.2 morphants (F). A–F: dorsal view; G–P lateral view. b: branchial arches; ep: endodermal pouches; h: hyoid cartilage; m: Meckel′s cartilage; ov: otic vesicle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx2a WT standard conditions 10-13 somites cranial neural crest cell ISH
20-25 somites pharyngeal arch 1 cranial neural crest ISH
20-25 somites pharyngeal arch 2 cranial neural crest ISH
20-25 somites pharyngeal arch 3-7 cranial neural crest ISH
Prim-5 pharyngeal arch 1 cranial neural crest ISH
Prim-5 pharyngeal arch 2 cranial neural crest ISH
Prim-5 pharyngeal arch 3-7 cranial neural crest ISH
Long-pec pharyngeal arch 1 cranial neural crest ISH
Long-pec pharyngeal arch 2 cranial neural crest ISH
Long-pec pharyngeal arch 3-7 cranial neural crest ISH
WT + MO1-pknox1.2 standard conditions 10-13 somites cranial neural crest cell ISH
20-25 somites pharyngeal arch 1 cranial neural crest ISH
20-25 somites pharyngeal arch 2 cranial neural crest ISH
20-25 somites pharyngeal arch 3-7 cranial neural crest ISH
Prim-5 pharyngeal arch 1 cranial neural crest ISH
Prim-5 pharyngeal arch 2 cranial neural crest ISH
Prim-5 pharyngeal arch 3-7 cranial neural crest ISH
Long-pec pharyngeal arch 1 cranial neural crest ISH
Long-pec pharyngeal arch 2 cranial neural crest ISH
Long-pec Not Detected pharyngeal arch 3-7 cranial neural crest ISH
fgf3 WT standard conditions Long-pec pharyngeal pouch 1 ISH
Long-pec pharyngeal pouch 2 ISH
Long-pec pharyngeal pouch 3 ISH
Long-pec pharyngeal pouch 4 ISH
Long-pec pharyngeal pouch 5 ISH
WT + MO1-pknox1.2 standard conditions Long-pec pharyngeal pouch 1 ISH
Long-pec pharyngeal pouch 2 ISH
foxd3 WT standard conditions 10-13 somites neural crest cell ISH
WT + MO1-pknox1.2 standard conditions 10-13 somites neural crest cell ISH
GFP ia1Tg standard conditions Prim-25 pharyngeal pouch 1 IFL
Prim-25 pharyngeal pouch 2 IFL
Prim-25 pharyngeal pouch 3 IFL
Prim-25 pharyngeal pouch 4 IFL
Prim-25 pharyngeal pouch 5 IFL
ia1Tg + MO1-pknox1.2 standard conditions Prim-25 pharyngeal pouch 1 IFL
Prim-25 pharyngeal pouch 2 IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-5 IHC WT standard conditions Prim-25 pharyngeal pouch 1
IHC Prim-25 pharyngeal pouch 2
IHC Prim-25 pharyngeal pouch 3
IHC Prim-25 pharyngeal pouch 4
IHC Prim-25 pharyngeal pouch 5
IHC WT + MO1-pknox1.2 standard conditions Prim-25 pharyngeal pouch 1
IHC Prim-25 pharyngeal pouch 2
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-pknox1.2 standard conditions Prim-25 pharyngeal pouch 3 aplastic, abnormal
Prim-25 pharyngeal pouch 4 aplastic, abnormal
Prim-25 pharyngeal pouch 5 aplastic, abnormal
Prim-25 pharyngeal system development process quality, abnormal
Long-pec endoderm development process quality, abnormal
Long-pec neural crest cell differentiation process quality, abnormal
Long-pec pharyngeal pouch 3 aplastic, abnormal
Long-pec pharyngeal pouch 4 aplastic, abnormal
Long-pec pharyngeal pouch 5 aplastic, abnormal
Long-pec pharyngeal system development process quality, abnormal
ia1Tg + MO1-pknox1.2 standard conditions Prim-25 endoderm development process quality, abnormal
Prim-25 pharyngeal pouch 3 aplastic, abnormal
Prim-25 pharyngeal pouch 4 aplastic, abnormal
Prim-25 pharyngeal pouch 5 aplastic, abnormal
Prim-25 pharyngeal system development process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 343(1-2), Vaccari, E., Deflorian, G., Bernardi, E., Pauls, S., Tiso, N., Bortolussi, M., and Argenton, F., prep1.2 and aldh1a2 participate to a positive loop required for branchial arches development in zebrafish, 94-103, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.