FIGURE

Fig. S6

ID

ZDB-FIG-100622-14

Publication

Devireddy et al., 2010 - A mammalian siderophore synthesized by an enzyme with a bacterial homolog involved in enterobactin production

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Fig. S6

Additional Experiments, Related to Figure 7

(A) bdh2 is broadly expressed in zebrafish embryos. Whole-mount in situ hybridization analysis reveals that at 28 hr post fertilization (hpf) bdh2 mRNA is widely distributed throughout the zebrafish embryo. As a negative control, in situ hybridization was also performed using a bdh2 sense-strand probe (bottom).

(B) Confirmation of decreased hemoglobin levels in Bdh2-depleted zebrafish embryos using a second, unrelated bdh2 MO. (Top) Phenotypic analysis of control MO- and bdh2 MO-injected embryos at 72 hpf. (Bottom) Whole-mount o-dianisidine staining of control MO and bdh2 MO-injected embryos at 72 hpf. Shown are lateral (top) and ventral (bottom) views of the anterior region of embryos.

Expression Data

Gene:	bdh2
Fish:	WT
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-bdh2
Knockdown Reagent:	MO2-bdh2
Observed In:	hemoglobin biosynthetic process nucleate erythrocyte hemoglobin complex
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 141(6), Devireddy, L.R., Hart, D.O., Goetz, D.H., and Green, M.R., A mammalian siderophore synthesized by an enzyme with a bacterial homolog involved in enterobactin production, 1006-1017, Copyright (2010) with permission from Elsevier. Full text @ Cell